E-Cadherin, Integrin Alpha2 (Cd49b), and Transferrin Receptor-1 (Tfr1) Are Promising Immunohistochemical Markers of Selected Adverse Pathological Features in Patients Treated with Radical Prostatectomy

Autor:	Piotr Zapała, Karolina Garbas, Zbigniew Lewandowski, Barbara Górnicka, Piotr Radziszewski, Łukasz Fus, Łukasz Zapała
Jazyk:	angličtina
Rok vydání:	2021
Předmět:	Oncology medicine.medical_specialty PTEN TfR1 extraprostatic extension of prostate cancer medicine.medical_treatment Transferrin receptor Article nomogram Prostate cancer PSA heregulin Internal medicine Medicine biology business.industry Cadherin Prostatectomy nodal involvement of prostate cancer Retrospective cohort study General Medicine Nomogram medicine.disease CD49b NF2 biology.protein Immunohistochemistry e-cadherin business
Zdroj:	Journal of Clinical Medicine; Volume 10; Issue 23; Pages: 5587 Journal of Clinical Medicine Journal of Clinical Medicine, Vol 10, Iss 5587, p 5587 (2021)
ISSN:	2077-0383
DOI:	10.3390/jcm10235587
Popis:	In patients treated for prostate cancer (PCa) with radical prostatectomy (RP), determining the risk of extraprostatic extension (EPE) and nodal involvement (NI) remains crucial for planning nerve-sparing and extended lymphadenectomy. The study aimed to determine proteins that could serve as immunohistochemical markers of locally advanced PCa. To select candidate proteins associated with adverse pathologic features (APF) reverse-phase protein array data of 498 patients was retrieved from The Cancer Genome Atlas. The analysis yielded 6 proteins which were then validated as predictors of APF utilizing immunohistochemistry in a randomly selected retrospective cohort of 53 patients. For univariate and multivariate analysis, logistic regression was used. Positive expression of TfR1 (OR 13.74; p = 0.015), reduced expression of CD49b (OR 10.15; p = 0.013), and PSA (OR 1.29; p = 0.013) constituted independent predictors of EPE, whereas reduced expression of e-cadherin (OR 10.22; p = 0.005), reduced expression of CD49b (OR 24.44; p = 0.017), and PSA (OR 1.18; p = 0.002) were independently associated with NI. Both models achieved high discrimination (AUROC 0.879 and 0.888, respectively). Immunohistochemistry constitutes a straightforward tool that might be easily utilized before RP. Expression of TfR1 and CD49b is associated with EPE, whereas expression of e-cadherin and CD49b is associated with NI. Since following immunohistochemical markers predicts respective APFs independently from PSA, in the future they might supplement existing preoperative nomograms or be implemented in novel tools.
Databáze:	OpenAIRE
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