Homogeneous assays using aptamers
| Autor: | Audrey Sassolas, Béatrice D. Leca-Bouvier, Loïc J. Blum |
|---|---|
| Přispěvatelé: | Institut de Chimie et Biochimie Moléculaires et Supramoléculaires (ICBMS), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut de Chimie du CNRS (INC)-École Supérieure Chimie Physique Électronique de Lyon-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2011 |
| Předmět: |
Aptamer
Molecular Sequence Data MESH: Aptamers Nucleotide Deoxyribozyme Fluorescence spectrometry Analytical chemistry Nanoparticle Biosensing Techniques MESH: Base Sequence 01 natural sciences Biochemistry Fluorescence MESH: Magnetic Resonance Imaging Analytical Chemistry 03 medical and health sciences chemistry.chemical_compound MESH: RNA Catalytic MESH: Colorimetry Electrochemistry Animals Humans Environmental Chemistry RNA Catalytic MESH: Animals [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry Molecular Biology/Biochemistry [q-bio.BM] Spectroscopy 030304 developmental biology 0303 health sciences MESH: Humans MESH: Molecular Sequence Data Base Sequence biology Chemistry Oligonucleotide MESH: Fluorescence 010401 analytical chemistry Ribozyme Electrochemical Techniques Aptamers Nucleotide Magnetic Resonance Imaging Combinatorial chemistry MESH: Electrochemical Techniques 0104 chemical sciences biology.protein Colorimetry MESH: Biosensing Techniques DNA |
| Zdroj: | Analyst Analyst, Royal Society of Chemistry, 2011, 136 (2), pp.257-74. ⟨10.1039/c0an00281j⟩ |
| ISSN: | 1364-5528 0003-2654 |
| Popis: | International audience; Aptamers are DNA or RNA oligonucleotides that can bind with high affinity and specificity to a wide range of targets such as proteins, metal ions or pathogenic microorganisms. Soluble aptamers and aptazymes have been used as sensing elements for developing homogeneous assays in a solution phase, the whole sensing process being carried out in a homogeneous solution. Contrary to most conventional heterogeneous assays that are time-consuming and labor-intensive, aptamer-based homogeneous assays are simple, easy-to-perform, rapid and do not require immobilization nor washing steps. To our knowledge, this review is the first entirely dedicated to aptamer-based homogeneous assays. Optical detection appears as the most developed technique. Colorimetry represents the simplest sensing mode that occupies a very important position among aptamer-based assays, involving gold nanoparticle aggregation (with unmodified or aptamer-modified gold NPs), the formation of HRP-mimicking DNAzyme with hemin, dye displacement or interactions with a cationic polymer. Fluorescence that is highly sensitive offers the most developed detection mode. Aptamers can be labeled or not, to give rise to turn-on or usually less sensitive turn-off fluorescent assays. Newly reported and thus less developed non-conventional magnetic resonance imaging (MRI) and electrochemistry also recently appeared in the literature, thrombin still remains the main detected target. Homogeneous assays based on aptazyme, an aptamer sequence connected to a known ribozyme motif, are also described in this review, involving optical detection, by colorimetry or fluorescence. |
| Databáze: | OpenAIRE |
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