Loss of heterozygosity at 12p13 and loss of p27KIP1 protein expression contribute to melanoma progression
| Autor: | Sebastian Hauser, Joachim Woenckhaus, Christian Woenckhaus, Klaus Failing, Thomas Dittberner, Micaela Poetsch, Jürgen Giebel, Irina Fenic |
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| Rok vydání: | 2004 |
| Předmět: |
medicine.medical_specialty
Tumor suppressor gene Loss of Heterozygosity Biology Pathology and Forensic Medicine Loss of heterozygosity Exon medicine Humans Melanoma Molecular Biology Chromosomes Human Pair 12 Intracellular Signaling Peptides and Proteins Anatomical pathology Cell Biology General Medicine medicine.disease Immunohistochemistry Tumor progression Disease Progression Cancer research CDKN1B Carrier Proteins Cyclin-Dependent Kinase Inhibitor p27 Microsatellite Repeats |
| Zdroj: | Virchows Archiv. 445:491-497 |
| ISSN: | 1432-2307 0945-6317 |
| DOI: | 10.1007/s00428-004-1049-6 |
| Popis: | Little is known about the mechanisms causing p27KIP1 decrease in melanomas. Therefore, we performed loss of heterozygosity (LOH) analysis with polymerase chain reaction at seven different loci surrounding the p27KIP1/CDKN1B gene at 12p13 and direct DNA sequencing analysis of all exons. Furthermore, the immunohistochemical expression of p27KIP1 and Ki-67 was investigated. Only two mutations in the sequence of p27KIP1/CDKN1B were detected, but the number of tumours showing LOH at 12p13 increased significantly with the parameters of tumour progression (pT level, P=0.018; Breslow index, P=0.01; Clark level, P |
| Databáze: | OpenAIRE |
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