Lysine Decarboxylase Activity in Broth and Agar Media
| Autor: | Harry R. Elston |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1971 |
| Předmět: |
Bacilli
food.ingredient Carboxy-Lyases Lysine Color Lactose complex mixtures General Biochemistry Genetics and Molecular Biology Microbiology chemistry.chemical_compound food Enterobacteriaceae Species Specificity Agar Food science General Pharmacology Toxicology and Pharmaceutics Bacteriological Techniques General Immunology and Microbiology Lysine decarboxylase biology Bacteria General Medicine biology.organism_classification equipment and supplies Culture Media chemistry Pseudomonas aeruginosa bacteria Fermentation Indicators and Reagents Clinical Microbiology Virology and Immunology |
| Popis: | Four lysine decarboxylase media were studied by testing them with 305 Enterobacteriaceae and 42 nonfermenting bacilli. A comparison was made between lysine decarboxylase broth medium (Moeller base) and Johnson's semisolid agar without lactose and Bachrach's broth medium and lysine-agar slants which contain lactose. The nonlactose media, lysine decarboxylase broth and the semisolid medium of Johnson, were the best media for use with all of the bacteria studied. The exclusion of lactose from lysine decarboxylase medium seems desirable to extend the usefulness of this medium among members of the Enterobacteriaceae. When the results with lysine decarboxylase broth and Johnson's semisolid medium without lactose were compared, a 6% difference existed between the results obtained with lysine decarboxylase broth and Johnson's semisolid agar. When the results with Bachrach's broth and lysine-agar slants with lactose were compared, a 1% difference existed between Bachrach's broth and the agar slant method. At times, reading and interpretation were difficult because of intermediate degrees of color change. The inability of Pseudomonas aeruginosa or Herellea to utilize glucose under the anaerobic condition of the medium makes the lysine decarboxylase test an undesirable procedure for these organisms. Of the four test media used, the lysine-lactose-agar slants seemed to be the least desirable because of the more frequent occurrence of indistinct color reactions and shifts in color. |
| Databáze: | OpenAIRE |
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