Expression of monocyte chemoattractant protein-1 in monocytes and effects of native and oxidized very low density lipoproteins
| Autor: | Deng Zhongduan, Wang Guo-ping, Ni Juan |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Very low-density lipoprotein
Arteriosclerosis Chemistry Mrna expression Monocyte Total rna nutritional and metabolic diseases Chemotaxis Lipoproteins VLDL Molecular biology Monocytes medicine.anatomical_structure Biochemistry Management of Technology and Innovation medicine Humans lipids (amino acids peptides and proteins) RNA Messenger Northern blot Oxidation-Reduction Incubation Chemokine CCL2 Monocyte chemoattractant protein |
| Zdroj: | Journal of Tongji Medical University. 17:203-205 |
| ISSN: | 0257-716X |
| DOI: | 10.1007/bf02895619 |
| Popis: | Monocyte chemoattractant protein-1 (MCP-1), a potent chemoattractant, is thought to play an important role in migration of monocytes into atherosclerotic lesions. The present study was designed to investigate the capacity of human peripheral blood monocytes to express MCP-1 and effects of native very low density lipoprotein (VLDL) and oxidized VLDL (OX-VLDL) on the expression. The total RNA was extracted from cultured monocytes, which were exposed to VLDL and OX-VLDL, and the media conditioned by monocytes were collected. MCP-1 mRNA expression was examined by Northern blot analysis. MCP-1 protein in conditioned media was determined by using sandwich ELISA. The results showed that monocytes can express MCP-1 after a 24 h incubation at 37 degrees C, and the expression was markedly increased by a exposure to OX-VLDL, whereas the expression was slightly increased when exposed to VLDL. It suggests that the capacity of monocytes to produce MCP-1 that recruits and activates circulating monocytes may be of considerable importance in atherogenesis, and oxidation of VLDL enhances its potential to promote atherogenesis. |
| Databáze: | OpenAIRE |
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