The delayed cytotoxic effect of 6-mercaptopurine Characterization of the unbalanced growth in HeLa cells produced by 6-mercaptopurine
| Autor: | Alan R. P. Paterson, J. Navarova, Katarína Horáková |
|---|---|
| Rok vydání: | 1974 |
| Předmět: |
Programmed cell death
Time Factors Lysis Cell Survival Mitosis Biochemistry Genetics and Molecular Biology (miscellaneous) Chromosomes HeLa chemistry.chemical_compound medicine Humans Cytotoxic T cell RNA Neoplasm Cell Nucleus biology Mercaptopurine Demecolcine DNA Neoplasm biology.organism_classification Neoplasm Proteins Cell biology chemistry Nucleic acid Cell Division DNA HeLa Cells medicine.drug |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis. 366:333-340 |
| ISSN: | 0005-2787 |
| Popis: | Pulse exposure to 6-mercaptopurine induced a delayed cytotoxic reaction in HeLa cells at concentrations 10–200 μM. Abortive proliferation occurred after lethal 6-mercaptopurine exposure. Unbalanced growth was an integral part of this delayed reaction. In connection with decrease in cell proliferation which followed 6-mercaptopurine treatment a gradual increase in cell size occurred. The initial aspect of the 6-mercaptopurine delayed reaction was characterized by decreases in DNA and RNA content per unit cell volume, while protein concentrations remain constant. However, in the later post-6-mercaptopurine exposure period the protein:cell volume ratios increased and nucleic acids accumulated. The increased content of DNA suggested that cells synthesized DNA without entering into mitosis and that dying cells were in late-S or G2 phases prior to lysis. Thus, cell death was preceded by disturbance of cellular, nuclear and chromosomal divisions. |
| Databáze: | OpenAIRE |
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