Cyclodextrin Production by Bacillus lehensis Isolated from Cassava Starch: Characterisation of a Novel Enzyme
| Autor: | Mary Helen Palmuti Braga Vettori, Kate Cristina Blanco, F. F. de Moraes, Jonas Contiero, Rubens Monti, Natalia Sozza Bernardi |
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| Přispěvatelé: | Universidade Estadual Paulista (Unesp), Universidade Estadual de Maringá (UEM) |
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
0301 basic medicine
chemistry.chemical_classification Chromatography Cyclodextrin biology purification Starch Cyclodextrin glycosyltransferase Enzyme assay 03 medical and health sciences chemistry.chemical_compound affinity chromatography 030104 developmental biology Monomer Enzyme chemistry Affinity chromatography cyclodextrin glycosyltransferase Protein purification biology.protein Food Science |
| Zdroj: | Web of Science Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| Popis: | Made available in DSpace on 2014-12-03T13:11:02Z (GMT). No. of bitstreams: 0 Previous issue date: 2014-01-01Bitstream added on 2014-12-03T13:22:49Z : No. of bitstreams: 1 WOS000332595800007.pdf: 696310 bytes, checksum: 6db35f94bfa76b161d78680a96b40225 (MD5) The properties of a previously unknown enzyme, denominated cyclodextrin glycosyltransferase, produced from Bacillus lehensis, were evaluated using affinity chromatography for protein purification. Enzyme characteristics (optimum pH and temperature; pH and temperature stability), the influence of substances on the enzyme activity, enzyme kinetics, and cyclodextrin production were analysed. Cyclodextrin glycosyltransferase was purified up to 320.74-fold by affinity chromatography using beta-cyclodextrin as the binder and it exhibited 8.71% activity recovery. This enzyme is a monomer with a molecular weight of 81.27 kDa, as estimated by SDS-PAGE. Optimum temperature and pH for cydodextrin glycosyltransferase were 55 degrees C and 8.0, respectively. The Michaelis-Menten constant was 8.62 g/l during maximum velocity of 0.858 g/l.h. UNESP Univ Estadual Paulista, Inst Biol Sci, Dept Biochem & Microbiol, BR-1515 Rio Claro, SP, Brazil UEM Univ Estadual Maringa, Dept Chem Engn, Maringa, Parana, Brazil UNESP Univ Estadual Paulista, Fac Pharmaceut Sci, Dept Food & Nutr, BR-1515 Rio Claro, SP, Brazil UNESP Univ Estadual Paulista, Inst Biol Sci, Dept Biochem & Microbiol, BR-1515 Rio Claro, SP, Brazil UNESP Univ Estadual Paulista, Fac Pharmaceut Sci, Dept Food & Nutr, BR-1515 Rio Claro, SP, Brazil |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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