Establishment of an Anti-acne Vulgaris Evaluation Method Based on TLR2 and TLR4-mediated Interleukin-8 Production
| Autor: | Panida Laoratthaphong, Pithi Chanvorachote, Titiporn Tongyen, Pichit Suvanprakorn, Ornjira Prakhongcheep |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Keratinocytes
Lipopolysaccharides Cancer Research Lipopolysaccharide Cell Survival Receptor expression Peptidoglycan Pharmacology General Biochemistry Genetics and Molecular Biology Cell Line 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Acne Vulgaris Humans MTT assay Interleukin 8 Chemistry Interleukin-8 Toll-Like Receptor 2 Teichoic Acids Toll-Like Receptor 4 Zinc TLR2 030220 oncology & carcinogenesis TLR4 Lipoteichoic acid Research Article |
| Zdroj: | In Vivo. 33:1929-1934 |
| ISSN: | 1791-7549 0258-851X |
| Popis: | Background/aim To date, no cell-based assay that focuses on the prime cause of acne initiation through activation of toll-like receptor2 and 4 and interleukin-8 (IL-8) production exists. Herein, we present an assay that evaluates acne by determining TLR2 and 4 expression and activation. Materials and methods Viability of keratinocytes was determined by the MTT assay. IL-8 was evaluated by ELISA. Immunocytochemistry was performed for determining receptor expression. Results Lipoteichoic acid (LTA), peptidoglycan (PGN) and lipopolysaccharide (LPS) induced IL-8 production. Pre-treatment of cells with TLR2 and TLR4 inhibitors, before stimulation, reduced IL-8 production. Zinc gluconate was used for verification. Zinc can significantly suppress IL-8 production in the system. Treatment of cells with LTA+PGN or LPS resulted in increased TLR2 and TLR4 expression on the cell surface. This effect was prevented by zinc treatment. Conclusion The measurement of IL-8 and TLR2 and TLR4 levels can be used for the evaluation of anti-acne treatment. |
| Databáze: | OpenAIRE |
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