Progress in the development of Fasciola hepatica vaccine using recombinant fatty acid binding protein with the adjuvant adaptation system ADAD
| Autor: | María Sol Arias, Julio López-Abán, Patrocinio Morrondo, Pablo Díez-Baños, Juan José Nogal, George V. Hillyer, P. Casanueva, Antonio Muro, A.R. Martínez-Fernández |
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| Rok vydání: | 2007 |
| Předmět: |
Fascioliasis
Time Factors Chemistry Pharmaceutical medicine.medical_treatment Sheep Diseases Fatty Acid-Binding Proteins Fatty acid-binding protein Microbiology Mice Antigen Hepatica parasitic diseases medicine Animals Fasciola hepatica Fasciolosis Mice Inbred BALB C Vaccines Sheep General Veterinary biology Quillaja saponaria General Medicine medicine.disease biology.organism_classification Virology Recombinant Proteins biology.protein Female Parasitology Antibody Adjuvant |
| Zdroj: | Veterinary Parasitology. 145:287-296 |
| ISSN: | 0304-4017 |
| DOI: | 10.1016/j.vetpar.2006.12.017 |
| Popis: | Fatty acid binding proteins (FABP) have been designed as a potential vaccine against fasciolosis. In this work, the immunoprophylaxis of the recombinant Fh15 FABP from F. hepatica (Fh15) in adjuvant/immunomodulator ADAD system was evaluated using mice and sheep challenged with F. hepatica. The ADAD system combines the Fh15 antigen with an immunomodulator (hydroalcoholic extract of Polypodium leucotomos; PAL) and/or an adjuvant (saponins of Quillaja saponaria; Qs) in a water/oil emulsion (30/70) with a non-mineral oil (Montanide). All the infected control mice died by 41–48 days post-infection. The mice vaccinated with ADAD only with PAL + Fh15 present a survival rate of 40–50% and those vaccinated with ADAD containing PAL + Qs + Fh15 had a survival rate of 50–62.5%. IgG1 antibodies were lower in surviving mice in comparison with non-surviving mice. The sheep vaccinated with ADAD PAL + Qs + Fh15 showed lower fluke recovery (43%), less hepatic lesions and higher post-infection daily weight gain than F. hepatica infected control animals. Thus, the ADAD system using recombinant fatty acid binding proteins from F. hepatica could be a good option to develop vaccines against F. hepatica. |
| Databáze: | OpenAIRE |
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