The Effect of Estrogen and Progesterone on the Level of Amidase Activity in Fluid Flushed from the Uteri of Ovariectomized Mice
| Autor: | R. C. Hoversland, H. M. Weitlauf |
|---|---|
| Rok vydání: | 1978 |
| Předmět: |
medicine.medical_specialty
medicine.drug_class Peptide Baseline level Biology Amidohydrolases Substrate Specificity Amidase Mice Hormone regulation Internal medicine medicine Amidase activity Animals Chymotrypsin Castration Uterine lumen Progesterone chemistry.chemical_classification Estradiol Uterus Cell Biology General Medicine Endocrinology Reproductive Medicine chemistry Estrogen Ovariectomized rat Female |
| Zdroj: | Biology of Reproduction. 19:908-912 |
| ISSN: | 1529-7268 0006-3363 |
| DOI: | 10.1095/biolreprod19.4.908 |
| Popis: | The effect of estrogen and progesterone on the level of chymotrypsin-like amidase activity was estimatedin fluidflushedfrom the uteri of ovariectomized mice by hydrolysis of the peptide homolog glutarl-L-phenylalanyl-j3-naphthylamide (GPAN). Mice were ovariectomized and injected with either progesterone alone, progesterone followed by a combination of estrogen and progesterone or estrogen alone. The level of activity was found to be low and constant for several days in animals receiving progesterone alone; the addition of estrogen caused an increase in activity that reached maximum in 18 h and then returned to the baseline level. In contrast, the level of amidase activity increased and remained elevated following the injection of estrogen alone. A similar experiment was done with a-N-benzoyl-DL-arginine-s3-naphthylamide HCI (BANA) to estimate the level of amidase with trypsin-like activity: no trypsin-like amidase was detected. These findings demonstrate that estrogen is capable of increasing chymotrypsin-like amidase activity in the uterine lumen and that progesterone alters the pattern of this response. The possible implications of hormone regulation of amidase activity in the uterine lumen is discussed with respect to normal and delayed implantation. |
| Databáze: | OpenAIRE |
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