Enhancement of single-walled carbon nanotube accumulation in glioma cells exposed to low-strength electric field: Promising approach in cancer nanotherapy
Autor: | Pavel M. Bulai, Yuliya Kunitskaya, Tatsiana Kulahava, Renata Karpicz, Lena Golubewa, Mikhail V. Shuba |
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Rok vydání: | 2020 |
Předmět: |
0301 basic medicine
Nanotube Biophysics Carbon nanotube Spectrum Analysis Raman Biochemistry law.invention Membrane Potentials 03 medical and health sciences symbols.namesake 0302 clinical medicine Electricity law Electric field Glioma Cell Line Tumor medicine Animals Nanotechnology Molecular Biology Carbon nanomaterials Chemistry Nanotubes Carbon Cell Membrane Cell Biology medicine.disease Electric Stimulation Raman microspectroscopy Rats 030104 developmental biology 030220 oncology & carcinogenesis symbols Raman spectroscopy Intracellular |
Zdroj: | Biochemical and biophysical research communications. 529(3) |
ISSN: | 1090-2104 |
Popis: | The objective of the study is to determine the patterns of regulation of single-walled carbon nanotube accumulation, distribution, and agglomeration in glioma cells exposed to an external electric field. C6 glioma cells were treated with 5 μg/ml DNA wrapped single-walled carbon nanotubes and exposed to bi-phasic electric pulses (6.6 V/m, 200 Hz, pulse duration 1 ms). Nanotube accumulation was determined by Raman microspectroscopy and their intracellular local concentration was evaluated using the G-band intensity in Raman spectra of single-walled carbon nanotubes. It was revealed that the low-frequency and low-strength electric field stimulation of glioma cells exposed to single-walled carbon nanotubes led to facilitation and, thus, to amplification of nanotube accumulation inside the cells. The number of nanotubes in intracellular agglomerates increased from (28.8 ± 13.1) un./agglom. and (84.0 ± 28.7) un./agglom. in control samples to (60.6 ± 21.4) un./agglom. and (184.2 ± 53.4) un./agglom. for 1 h and 2 h stimulation, respectively. Thus, the tumor exposure to an external electric field makes it possible to more effectively regulate the accumulation and distribution of carbon nanotubes inside glioma cells allowing to reduce the applied therapeutic doses of carbon nanomaterial delivered anticancer drugs. |
Databáze: | OpenAIRE |
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