Maelstrom regulates spermatogenesis of the silkworm, Bombyx mori
| Autor: | Ye Yu, Zulian Liu, Shuqing Chen, Jun Xu, Kai Chen, Yongping Huang, Anjiang Tan |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Male
0106 biological sciences Transgene Mutant Piwi-interacting RNA Apoptosis Biology Real-Time Polymerase Chain Reaction medicine.disease_cause 01 natural sciences Biochemistry Tight Junctions 03 medical and health sciences Bombyx mori medicine Animals RNA-Seq Axis specification Spermatogenesis Molecular Biology Gene 030304 developmental biology 0303 health sciences Mutation Base Sequence fungi Sex Determination Processes Bombyx biology.organism_classification Cell biology 010602 entomology Insect Science Insect Proteins |
| Zdroj: | Insect Biochemistry and Molecular Biology. 109:43-51 |
| ISSN: | 0965-1748 |
| DOI: | 10.1016/j.ibmb.2019.03.012 |
| Popis: | The spermatogenesis of animal is essential for the reproduction and a very large number of genes participate in this procession. The Maelstrom (Mael) is identified essential for spermatogenesis in both Drosophila and mouse, though the mechanisms appear to differ. It was initially found that Mael gene is necessary for axis specification of oocytes in Drosophila, and recent studies suggested that Mael participates in the piRNA pathway. In this study, we obtained Bombyx mori Mael mutants by using a binary transgenic CRISPR/Cas9 system and analyzed the function of Mael in B. mori, a model lepidopteran insect. The results showed that BmMael is not necessary for piRNA pathway in the ovary of silkworm, whereas it might be essential for transposon elements (TEs) repression in testis. The BmMael mutation resulted in male sterility, and further analysis established that BmMael was essential for spermatogenesis. The spermatogenesis defects occurred in the elongation stage and resulted in nuclei concentration arrest. RNA-seq and qRT-PCR analyses demonstrated that spermatogenesis defects were associated with tight junctions and apoptosis. We also found that BmMael was not involved in the silkworm sex determination pathway. Our data provide insights into the biological function of BmMael in male spermatogenesis and might be useful for developing novel methods to control lepidopteron pests. |
| Databáze: | OpenAIRE |
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