Osteosarcoma growth suppression by riluzole delivery via iron oxide nanocage in nude mice
| Autor: | Marian Raghubir, Hiroshi Matsui, Shahana S. Mahajan, Justin Fang, Chowdhury Nowshin Rahman |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Male
0301 basic medicine Cancer Research Cell Survival Drug Compounding Metal Nanoparticles Mice Nude Bone Neoplasms Ferric Compounds Mice 03 medical and health sciences 0302 clinical medicine In vivo Cell Line Tumor osteosarcoma nanocage medicine Animals Humans cancer iron oxide nanoparticle xenograft Cell Proliferation Riluzole Oncogene Bone cancer Chemistry Cell Cycle Articles General Medicine medicine.disease Xenograft Model Antitumor Assays Molecular medicine Tumor Burden 3. Good health in vivo 030104 developmental biology Oncology Apoptosis 030220 oncology & carcinogenesis drug delivery Drug delivery Cancer research Osteosarcoma Injections Intraperitoneal medicine.drug |
| Zdroj: | Oncology Reports |
| ISSN: | 1791-2431 1021-335X |
| DOI: | 10.3892/or.2019.7420 |
| Popis: | Osteosarcomas are the most commonly occurring malignant bone cancer in young individuals. The survival rate of patients with metastatic osteosarcoma is low and has been stagnant for over two decades. We previously demonstrated that the glutamate release inhibitor, riluzole inhibits osteosarcoma cell growth. Towards the development of more effective therapy, we investigated the delivery of riluzole in human metastatic osteosarcoma xenografts in mice. We compared the efficacy of riluzole delivery by intraperitoneally injecting either free riluzole or riluzole released via two different shapes of iron oxide nanoparticles (nanocage or nanosphere) of size 15±2.5 nm. We monitored tumor size using Vernier calipers and bioluminescence assay and found a significant reduction in tumor size in the riluzole-treated groups when injected, either in free form or via nanoparticles, compared to the control groups (PBS, nanosphere or nanocage). Importantly, nanocage-delivered riluzole was most effective in reducing tumor size in the xenograft nude mice. While riluzole delivery induced apoptosis in tumor tissues in all three groups of riluzole-treated animals, it was highest in tumors from the nanocage-delivered riluzole group. Therefore, we conclude that riluzole is an effective drug to reduce tumor size in osteosarcoma and the efficacy of riluzole as a apoptotic and tumor-reducing drug is enhanced when delivered via nanocage. |
| Databáze: | OpenAIRE |
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