A Non-Chromatographic Non-Extraction Radioimmunoassay for Serum Aldosterone
| Autor: | Nishi K, Kazushige Iinuma, Yukinobu Arakawa, Atsushi Takagi, Kiyoshi Miyai, Kunio Kurata, Yuichi Kumahara, Toshio Ogihara |
|---|---|
| Rok vydání: | 1977 |
| Předmět: |
medicine.medical_specialty
Aldosterone Chromatography Endocrinology Diabetes and Metabolism Coefficient of variation Biochemistry (medical) Clinical Biochemistry Extraction (chemistry) Radioimmunoassay Polyethylene glycol Biochemistry Blood proteins chemistry.chemical_compound Endocrinology Blood serum chemistry Internal medicine medicine Humans Dichloromethane |
| Zdroj: | The Journal of Clinical Endocrinology & Metabolism. 45:726-731 |
| ISSN: | 1945-7197 0021-972X |
| DOI: | 10.1210/jcem-45-4-726 |
| Popis: | A direct radioimmunoassay for serum aldosterone was developed using a highly specific sntibody and 8-anilino-1-naphthalene sulfonic acid as a blocking agent to inhibit the binding of aldosterone to serum proteins. 125I-labeled aldosterone was used as the labeled antigen and polyethylene glycol was used to separate antibody-bound and free aldosterone. The minimum detectable concentration was 1.5 pg/tube. There were excellent correlations between the present method and other methods, i.e., 1) a method using tritiated aldosterone, 2) a method using dichloromethane extraction before assay, and 3) a commercial kit method. The intra-assay coefficient of variation was 6.9%, and the inter-assay coefficient of variation was 10.7%. The values found in normal human serum were comparable with those reported using other methods. The present radioimmunoassay eliminates both extraction of aldosterone from serum and chromatographic separation and requires only 0.1 ml of serum for assay. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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