The spindle assembly checkpoint functions during early development in non-chordate embryos
| Autor: | Stefania Castagnetti, Marianne Roca, Elisabeth Christians, Alex McDougall, Lydia Besnardeau, Janet Chenevert, Dalileh Nabi, Antonella Ruggiero, Richard R. Copley |
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| Přispěvatelé: | Laboratoire de Biologie du Développement de Villefranche sur mer (LBDV), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut de la Mer de Villefranche (IMEV), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), University of Aberdeen, Stazione Zoologica Anton Dohrn (SZN), Centre de biologie du développement (CBD), Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre de Biologie Intégrative (CBI), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Embryo
Nonmammalian Mad2 animal structures Mad1 [SDV]Life Sciences [q-bio] embryo Cell Cycle Proteins Spindle Apparatus [SDV.BC]Life Sciences [q-bio]/Cellular Biology [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC] Biology Microtubules Article Chromosome segregation 03 medical and health sciences 0302 clinical medicine Microtubule Chromosome Segregation Animals Kinetochores lcsh:QH301-705.5 [SDV.BDD]Life Sciences [q-bio]/Development Biology Mitosis 030304 developmental biology mitosis 0303 health sciences Kinetochore Nocodazole Embryogenesis Cell Cycle Checkpoints General Medicine Invertebrates Embryonic stem cell Cell biology Spindle apparatus Spindle checkpoint lcsh:Biology (General) embryonic structures M Phase Cell Cycle Checkpoints spindle checkpoint chordates 030217 neurology & neurosurgery Signal Transduction |
| Zdroj: | Cells Volume 9 Issue 5 Cells, MDPI, 2020, 9 (5), pp.1087. ⟨10.3390/cells9051087⟩ Cells, Vol 9, Iss 1087, p 1087 (2020) |
| ISSN: | 2073-4409 |
| DOI: | 10.1101/582759 |
| Popis: | In eukaryotic cells, a spindle assembly checkpoint (SAC) ensures accurate chromosome segregation. This control mechanism monitors proper attachment of chromosomes to spindle microtubules and delays mitotic progression if connections are erroneous or absent. The SAC operates in all eukaryotic cells tested so far, but is thought to be relaxed during early embryonic development in animals. Here, we evaluate the checkpoint response to lack of kinetochore-spindle microtubule interactions in early embryos of diverse animal species from the main metazoan groups. Our analysis shows that there are two classes of embryos, either proficient or deficient for SAC activation during cleavage. Sea urchins, mussels and jellyfish embryos show a prolonged mitotic block in the absence of spindle microtubules from the first cleavage division, while ascidian and amphioxus embryos, like those of Xenopus and zebrafish, continue mitotic cycling without delay. SAC competence during early development shows no correlation with cell size, chromosome number or kinetochore to cell volume ratio, ruling out the hypothesis that lack of checkpoint activity in early embryos is due to the large egg volume. Our results instead indicate that there is no inherent incompatibility between SAC activity and large fast-dividing embryonic cells. We suggest that SAC proficiency is the default situation of metazoan embryos, and that SAC activity is specifically silenced in chordate species with fast dividing embryos. |
| Databáze: | OpenAIRE |
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