Cytotoxic activity of lymphoid cells from mice immunized with semiallogeneic hybrid cells: Requirement of in vitro lymphoid cells culture for expression of cytotoxicity against a syngeneic chemically induced tumor
| Autor: | Béatrice Payelle, Anne-Françoise Goguel, Marie-France Poupon, Geneviève Lespinats |
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| Rok vydání: | 1982 |
| Předmět: |
Cytotoxicity
Immunologic Male Fibrosarcoma Immunology Hybrid Cells Biology Lymphocyte Activation Epitopes Mice Immune system medicine Animals Ascitic Fluid Cytotoxic T cell Lymphocytes Cytotoxicity Cells Cultured Antiserum Mice Inbred C3H Lymphokine-activated killer cell medicine.disease In vitro Cell biology Mice Inbred C57BL Interleukin 12 Cancer research Female Methylcholanthrene T-Lymphocytes Cytotoxic |
| Zdroj: | Cellular Immunology. 74:383-393 |
| ISSN: | 0008-8749 |
| Popis: | Semiallogeneic somatic hybrid cells (AB2) derived from fusion of a C57B1/6 chemically induced fibrosarcoma (MCB6-1) and a fibroblastic cell (A9) of C3H origin were used to immunize C57B1/6 mice against the parental MCB6-1 tumor cells. In vitro immune lymphocytes were directly cytotoxic against AB2 hybrid cells and A9 allogeneic parental cells, but could not lyse the syngeneic MCB6-1 parental tumor cells. Nevertheless, after a 4-day culture of these immune lymphocytes, a cytotoxic activity against the syngeneic MCB6-1 tumor cells appeared; expression of such a cytotoxic activity did not require the presence of stimulator cells (mitomycin-treated MCB6-1 tumor cells) during the culture. This cytotoxicity is mediated by T cells, as it was completely abrogated by treatment with anti-Thy 1–2 antiserum and complement. These results suggest that a maturation or a differentiation of immune T lymphocytes occurs during in vitro culture, and is necessary for the expression of antitumor cytotoxicity. |
| Databáze: | OpenAIRE |
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