Sex steroid application reverses changes in rat castration cells: Unbiased stereological analysis
| Autor: | Nataša Ristić, Ivana Jarić, Milica Manojlović-Stojanoski, Branka Šošić-Jurjević, B. Filipović, Verica Milošević, Nataša Nestorović, Svetlana Trifunović |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Testosterone propionate medicine.medical_specialty gonadotropic cells orchidectomy Stereology General Biochemistry Genetics and Molecular Biology 03 medical and health sciences chemistry.chemical_compound Internal medicine estradiol medicine Testosterone lcsh:QH301-705.5 Estradiol Gonadotropic cells Orchidectomy 030104 developmental biology Castration Endocrinology chemistry lcsh:Biology (General) Sex steroid testosterone stereology Estradiol dipropionate General Agricultural and Biological Sciences Luteinizing hormone Hormone |
| Zdroj: | Archives of Biological Sciences, Vol 68, Iss 4, Pp 821-828 (2016) Archives of Biological Sciences |
| ISSN: | 1821-4339 0354-4664 |
| Popis: | The aim of the present study was to examine the morphometric characteristics of follicle-stimulating hormone (FSH) and luteinizing hormone (LH) immunoreactive cells in the pituitaries of orchidectomized (Orx) and Orx testosterone- or estradiol-treated rats. Adult male Orx Wistar rats, 2 weeks post operation, received estradiol dipropionate (E) or testosterone propionate (T) for 3 weeks. Both controls, sham-operated (So) and Orx rats, were injected with solvent, in the same regime. Changes in the volume of pars distalis , the volumes of individual FSH- and LH-labeled cells, their numerical density and number were determined by unbiased design-based stereology. The FSH and LH intracellular content was estimated by relative intensity of fluorescence (RIF). We observed that Orx caused hyperstimulation of gonadotropic cells. Their volume, volume density, number, numerical density and intracellular hormone content significantly increased in comparison to So controls. Compared to Orx controls, T caused a significant decrease in the volume and volume density of gonadotropic cells and immunoreactive FSH and LH content in their cytoplasm. The volume of the pars distalis , the numerical density and number of gonadotropic cells were not changed as compared to Orx controls. Estradiol treatment caused a significant increase in the volume of the pars distalis , decreases in cell volume, volume and numerical density of gonadotropic cells, and FSH and LH intracellular content in comparison to Orx controls. The number of FSH-labeled cells increased. In conclusion, both T and E reversed all of the examined parameters of gonadotropic cells of Orx rats to the level of So controls, except in number. DOI: 10.2298/ABS151201070N Key words: estradiol; gonadotropic cells; orchidectomy; stereology; testosterone Received: December 1, 2015; Revised: February 17, 2016; Accepted: February 18, 2016; Published online: August 17, 2016 How to cite this article: Nestorovic NM, Trifunovic SL, Jaric IM, Manojlovic-Stojanoski MN, Ristic NM, Filipovic BR, Sosic-Jurjevic BT, Milosevic VLj. Sex steroid application reverses changes in rat castration cells: Unbiased stereological analysis. Arch Biol Sci. 2016;68(4):821-8. |
| Databáze: | OpenAIRE |
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