Lymphoid hyperplasia in transgenic mice over-expressing a secreted form of the human interleukin-1beta gene product
| Autor: | Akerblad P, Mikael Dohlsten, Björkdahl O, Tomas Leanderson, Gjörloff-Wingren A |
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| Rok vydání: | 1999 |
| Předmět: |
Genetically modified mouse
Antigens Differentiation T-Lymphocyte CD4-Positive T-Lymphocytes Immunology Enzyme-Linked Immunosorbent Assay Mice Transgenic Lymphocyte Activation Lymphoid hyperplasia Immunoglobulin G Immunophenotyping Gene product Mice Immune system medicine Immunology and Allergy Animals Humans Protein Isoforms L-Selectin Lymph node Hyperplasia biology Flow Cytometry Molecular biology Immunohistochemistry Immunoglobulin A Mice Inbred C57BL Lymphatic system medicine.anatomical_structure Hyaluronan Receptors biology.protein Interleukin-2 Original Article Interleukin-4 Lymph Nodes Antibody medicine.symptom Spleen Interleukin-1 |
| Zdroj: | Immunology. 96(1) |
| ISSN: | 0019-2805 |
| Popis: | To evaluate the biological effects of over-expression of interleukin-1beta (IL-1beta) on the immune system we have generated transgenic mice, expressing the IL-1beta gene fused to a heterologous signal sequence under the control of the mouse immunoglobulin enhancer (Emu). A prominent hyperplasia and a disturbed microarchitecture of lymphoid tissues were observed in the transgenic mice. The CD4+ T cells in the hyperplastic lymphoid organs seemed to invade the majority of the lymphoid organs including B-cell restricted areas. Analysis of lymph node cells revealed an increased frequency of CD4+ CD44high CD62L- T cells and local secretion of IL-2 and IL-4, compatible with an elevated number of activated T cells. Furthermore, significant levels of human IL-1beta in sera and high concentrations of serum immunoglobulin G (IgG) were observed in the transgenic mice. The data suggest a role for IL-1beta in controlling lymphoid microarchitecture and, when over-expressed, breaking the threshold in T-helper-B-cell interaction. |
| Databáze: | OpenAIRE |
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