The effect of nitrate and ammonium concentrations on growth and alkaloid accumulation of Atropa belladonna hairy roots
| Autor: | Marc-André Fliniaux, Françoise Gillet, José Edmundo Nava Saucedo, Lamine Bensaddek |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Scopolamine
Bioengineering Plant Roots Applied Microbiology and Biotechnology chemistry.chemical_compound Tissue culture Murashige and Skoog medium Nitrate Atropa belladonna Botany medicine Ammonium Atropine Derivatives Chromatography High Pressure Liquid Hyoscyamine Nitrates Plants Medicinal biology Alkaloid General Medicine biology.organism_classification Belladonna Alkaloids Quaternary Ammonium Compounds Plants Toxic chemistry Solanaceae Biotechnology medicine.drug |
| Zdroj: | Journal of Biotechnology. 85:35-40 |
| ISSN: | 0168-1656 |
| Popis: | The growth, the alkaloid production, as well as the scopolamine/hyoscyamine ratio of two clones of belladonna hairy roots were studied. The effects of nitrate and ammonium concentrations on these cultures were investigated. A rise in ammonium concentration caused the decline of the hairy roots, while nitrate had a marked effect on the alkaloid content. The alkaloid production obtained with 15.8 mM of NO3- and 20.5 mM of NH4+ was 1.2-1.4 times higher than that obtained when the roots were grown in the standard Murashige and Skoog medium (MS medium, 39.5 mM of NO3- and 20.5 mM of NH4+). The nitrate and ammonium concentrations in the culture medium also had a strong influence on the scopolamine/hyoscyamine ratio. When nitrate or ammonium concentrations were raised, that ratio also was increased 2-3-fold. The hairy root clones originating from transformations with two distinct strains of Agrobacterium had similar responses. |
| Databáze: | OpenAIRE |
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