Maternal omega-3 intake differentially affects the endocannabinoid system in the progeny's neocortex and hippocampus: Impact on synaptic markers
| Autor: | Roberta Verde, Danyelly Bruneska Gondim Martins, Fernando G. de Mello, Maria das Graças Tavares-do-Carmo, Bruna Klippel Ferreira, Fabio Arturo Iannotti, Karla Yasmin Dias Fraga, Patricia Coelho de Velasco, Thaysa Walléria de Aragão Santos, Ricardo Augusto de Melo Reis, Raquel Maria Pereira Campos, Vincenzo Di Marzo, Belmira Lara da Silveira Andrade-da-Costa, Alinny Rosendo Isaac |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty Cerebral cortex Diet Endocannabinoid system Hippocampus Omega-3 Plasticity Offspring Endocrinology Diabetes and Metabolism Clinical Biochemistry Neocortex Biology Hippocampal formation Biochemistry 03 medical and health sciences 0302 clinical medicine Pregnancy Internal medicine Cortex (anatomy) Fatty Acids Omega-3 medicine Animals Molecular Biology Cells Cultured Nutrition and Dietetics Maternal Nutritional Physiological Phenomena Rats 030104 developmental biology medicine.anatomical_structure Endocrinology nervous system Animals Newborn Synapses Synaptophysin biology.protein Female 030217 neurology & neurosurgery Endocannabinoids |
| Zdroj: | The Journal of nutritional biochemistry 96 (2021). doi:10.1016/j.jnutbio.2021.108782 info:cnr-pdr/source/autori:Isaac A.R.; de Velasco P.C.; Fraga K.Y.D.; Tavares-do-Carmo M.D.G.; Campos R.M.P.; Iannotti F.A.; Verde R.; Martins D.B.G.; Santos T.A.; Ferreira B.K.; de Mello F.G.; Di Marzo V.; Andrade-da-Costa B.L.D.S.; de Melo Reis R.A./titolo:Maternal omega-3 intake differentially affects the endocannabinoid system in the progeny`s neocortex and hippocampus: Impact on synaptic markers/doi:10.1016%2Fj.jnutbio.2021.108782/rivista:The Journal of nutritional biochemistry/anno:2021/pagina_da:/pagina_a:/intervallo_pagine:/volume:96 |
| ISSN: | 1873-4847 |
| DOI: | 10.1016/j.jnutbio.2021.108782 |
| Popis: | Omega-3 (n-3) polyunsaturated fatty acids (PUFA) and the endocannabinoid system (ECS) modulate several functions through neurodevelopment including synaptic plasticity mechanisms. The interplay between n-3PUFA and the ECS during the early stages of development, however, is not fully understood. This study investigated the effects of maternal n-3PUFA supplementation (n-3Sup) or deficiency (n-3Def) on ECS and synaptic markers in postnatal offspring. Female rats were fed with a control, n-3Def, or n-3Sup diet from 15 days before mating and during pregnancy. The cerebral cortex and hippocampus of mothers and postnatal 1-2 days offspring were analyzed. In the mothers, a n-3 deficiency reduced CB1 receptor (CB1R) protein levels in the cortex and increased CB2 receptor (CB2R) in both cortex and hippocampus. In neonates, a maternal n-3 deficiency reduced the hippocampal CB1R amount while it increased CB2R. Additionally, total GFAP isoform expression was increased in both cortex and hippocampus in neonates of the n-3Def group. Otherwise, maternal n-3 supplementation increased the levels of n-3-derived endocannabinoids, DHEA and EPEA, in the cortex and hippocampus and reduced 2-arachidonoyl-glycerol (2-AG) concentrations in the cortex of the offspring. Furthermore, maternal n-3 supplementation also increased PKA phosphorylation in the cortex and ERK phosphorylation in the hippocampus. Synaptophysin immunocontent in both regions was also increased. In vitro assays showed that the increase of synaptophysin in the n-3Sup group was independent of CB1R activation. The findings show that variations in maternal dietary omega-3 PUFA levels may impact differently on the ECS and molecular markers in the cerebral cortex and hippocampus of the progeny. |
| Databáze: | OpenAIRE |
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