Apoptosis-related gene expression induced by Colombian propolis samples in canine osteosarcoma cell line
| Autor: | Oscar Julián Murillo, Karina Basso Santiago, Orlando Torres García, Dolly Patricia Pardo-Mora, José Maurício Sforcin, Jaime Fabian Cruz Uribe, Eliza de Oliveira Cardoso, Rafael María Gutiérrez, Mauricio Rey-Buitrago, Monica Losada-Barragán, Fernanda Lopes Conte, Bruno José Conti |
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| Přispěvatelé: | Universidad Antonio Nariño, Universidad Nacional de Colombia, Universidade Estadual Paulista (Unesp) |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Veterinary medicine
Cytotoxicity Apoptosis Caspase 8 SF1-1100 Propolis 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Annexin osteosarcoma SF600-1100 Cytotoxic T cell Viability assay Propidium iodide 030304 developmental biology Caspase-9 0303 health sciences Osteosarcoma General Veterinary biology apoptosis Molecular biology Animal culture propolis chemistry 030220 oncology & carcinogenesis biology.protein cytotoxicity Research Article |
| Zdroj: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP Veterinary World, Vol 14, Iss 4, Pp 964-971 (2021) Veterinary World |
| Popis: | Made available in DSpace on 2021-06-25T11:15:36Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-04-01 Background and Aim: Osteosarcoma (OSA) is the most common bone tumor in canines and humans. This study aimed to assess the cytotoxic and apoptotic effects of Colombian propolis samples on a canine OSA cell line (OSCA-8) by evaluating the expression of BCL-2, BAX, CASPASE 9, CASPASE 8, and TNFR1 genes involved in the apoptosis pathway. Materials and Methods: After treating the cells with five Colombian propolis samples (Usm, Met, Fus, Sil, and Caj), we evaluated cell viability and lactate dehydrogenase (LDH) release. Early and late apoptosis was determined by flow cytometry using annexin V/propidium iodide. Furthermore, the effects of three selected samples on gene expression were analyzed by real-time polymerase chain reaction. Results: The Colombian propolis samples reduced OSCA-8 cell viability and increased LDH release. All samples induced apoptosis significantly and upregulated BCL-2 and CASPASE 8 expression. Usm and Sil increased BAX expression, Met and Sil induced CASPASE 9 expression, and Usm increased TNFR1. Conclusion: Colombian propolis samples exhibited cytotoxic and apoptotic effects on canine OSA cells, and CASPASE 8 upregulation indicated apoptosis induction by the extrinsic pathway. Department of Animal Health Facultad de Medicina Veterinaria Universidad Antonio Nariño Department of Morphology Facultad de Medicina Universidad Nacional de Colombia Department of Chemical and Biological Sciences São Paulo State University (UNESP) Institute of Biosciences Department of Chemical and Biological Sciences São Paulo State University (UNESP) Institute of Biosciences |
| Databáze: | OpenAIRE |
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