Treatment of Shwachman Syndrome by Japanese Herbal Medicine (Juzen‐Taiho‐To): Stimulatory Effects of Its Fatty Acids on Hemopoiesis in Patients
| Autor: | Tianxue Fan, Masahiro Hirayama, Tomoko Iguchi‐Uehira, Yunze Cui, Yongan Li, Susumu Ikehara, Guo-Xiang Yang, Kikuya Sugiura, Haruki Yamada, Hiroko Hisha, Yohnosuke Kobayashi, Muneo Inaba, Urara Kohdera |
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| Rok vydání: | 2002 |
| Předmět: |
Male
medicine.medical_specialty Stromal cell Pancytopenia Linolenic acid Biology Neutropenia Umbilical cord Internal medicine medicine Humans Child Exocrine pancreatic insufficiency Bone Marrow Diseases Cells Cultured chemistry.chemical_classification Fatty Acids Pancreatic Diseases alpha-Linolenic Acid Fatty acid Recovery of Function Cell Biology Hematopoietic Stem Cells medicine.disease Hematopoiesis Haematopoiesis Treatment Outcome medicine.anatomical_structure Endocrinology chemistry Immunology Molecular Medicine Female Bone marrow Mitogens Cell Division Drugs Chinese Herbal Oleic Acid Developmental Biology |
| Zdroj: | STEM CELLS. 20:311-319 |
| ISSN: | 1549-4918 1066-5099 |
| Popis: | Juzen-taiho-to (a Japanese herbal medicine) has been traditionally administered to patients with anemia, neutropenia, or wasting syndrome. We previously attempted to isolate and purify the hemopoiesis-stimulatory components in Juzen-taiho-to extracts using an in vitro hemopoietic stem cell (HSC) assay method in which mouse HSCs can proliferate on a stromal cell line (MS-5). We have found that fatty acids (particularly oleic acid and linolenic acid) actively promote the proliferation of HSCs, and that the effect is mediated by stromal cells, rather than by any direct action on the HSCs. In the present study, we show, using human normal bone marrow cells (BMCs) and umbilical cord blood cells, that similar stimulatory effects are due to the presence of oleic acid and linolenic acid, which stimulate the proliferation of HSCs in stroma-based culture systems. Furthermore, a marked stimulatory effect was noted on BMCs from patients with Shwachman syndrome, which shows pancreatic and bone marrow dysfunctions. We also show the data on hemopoietic recovery after the administration of Juzen-taiho-to to a patient with Shwachman syndrome. These findings suggest that decreased fatty acid levels in the blood, caused by exocrine pancreatic insufficiency, induce bone marrow dysfunction in Shwachman syndrome. |
| Databáze: | OpenAIRE |
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