FT-IR microscopy characterization of sol–gel layers prior and after glucose oxidase immobilization for biosensing applications
| Autor: | Marianna Portaccio, N. Manolova, D.G. Mita, Olya Stoilova, Maria Lepore, I. Rashkov, B. Della Ventura |
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| Přispěvatelé: | Portaccio, Marianna Bianca Emanuela, B., DELLA VENTURA, D. G., Mita, N., Manolova, O., Stoilova, I., Rashkov, Lepore, Maria |
| Rok vydání: | 2010 |
| Předmět: |
Materials science
Immobilized enzyme biology Infrared Analytical chemistry Infrared spectroscopy 02 engineering and technology General Chemistry 010402 general chemistry 021001 nanoscience & nanotechnology Condensed Matter Physics 01 natural sciences Fluorescence 0104 chemical sciences Electronic Optical and Magnetic Materials Biomaterials Chemical engineering Microscopy Materials Chemistry Ceramics and Composites biology.protein Glucose oxidase Fourier transform infrared spectroscopy 0210 nano-technology Biosensor |
| Zdroj: | Journal of Sol-Gel Science and Technology |
| ISSN: | 1573-4846 0928-0707 |
| DOI: | 10.1007/s10971-010-2343-1 |
| Popis: | Micro-Attenuated Total Reflection (ATR) Fourier Transform Infrared spectroscopy was used to investigate sol–gel layers for biosensing applications prior and after glucose oxidase (GOD) immobilization. The changes occurring in sol–gel infrared spectrum after GOD immobilization were clearly evidenced confirming the retaining of the enzyme activity. Moreover, micro-ATR experimental technique allowed us to investigate the spatial distribution of enzyme concentration. The non-destructive nature of our approach also enabled to monitor the time stability of sol–gel layers and of embedded GOD. The temporal evolution of some peaks in infrared spectra of these sol–gel layers was compared with absorption and steady-state fluorescence measurements. The results reported here confirm that micro-ATR infrared spectroscopy can be usefully employed for a non- or minimally invasive detailed characterization of supports for enzyme immobilization. |
| Databáze: | OpenAIRE |
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