Tissue sites of catabolism of albumin in rabbits
| Autor: | Daniel Steinberg, W. F. Beltz, S. Yedgar, T E Carew, R. C. Pittman |
|---|---|
| Rok vydání: | 1983 |
| Předmět: |
Sucrose
Physiology Endocrinology Diabetes and Metabolism Serum albumin Kidney Feces chemistry.chemical_compound Albumins Physiology (medical) medicine Albuminuria Animals Tissue Distribution Carbon Radioisotopes Intestinal Mucosa Bovine serum albumin Trichloroacetic acid Serum Albumin biology Catabolism Muscles Feces analysis Albumin Kidney metabolism Organ Size Liver Biochemistry chemistry Isotope Labeling biology.protein Female Rabbits medicine.symptom |
| Zdroj: | American Journal of Physiology-Endocrinology and Metabolism. 244:E101-E107 |
| ISSN: | 1522-1555 0193-1849 |
| DOI: | 10.1152/ajpendo.1983.244.1.e101 |
| Popis: | The sites of albumin catabolism were determined in rabbits using [14C]sucrose-labeled rabbit albumin. The [14C]sucrose moiety is not degradable and accumulates in tissues degrading the protein. Albumin was labeled with [14C]sucrose, and the monomeric form was selected for injection into rabbits. The validity of the sucrose-labeled albumin as a tracer for native albumin was shown by the similar plasma decay kinetics of 125I-labeled albumin derivatized with sucrose and 131I-labeled native albumin and by the similar decay kinetics for the biologically screened and unscreened preparations of [14C]sucrose-albumin. Two days after injection of [14C]sucrose-albumin, tissues were assayed for accumulated 14C-labeled degradation products soluble in trichloroacetic acid. All tissues catabolized albumin with no tissue of predominant importance; liver, kidney, and muscle were the largest contributors. Expressed in terms of activity per unit wet weight, adrenal, kidney, spleen, ovary, bone marrow, and liver were the most active. These most active tissues are those with fenestrated or discontinuous capillary beds, suggesting that exposure to high concentrations of albumin is an important determinant of their high rates of albumin degradation. |
| Databáze: | OpenAIRE |
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