Nanoencapsulated hypericin in P-123 associated with photodynamic therapy for the treatment of dermatophytosis
| Autor: | Raquel da Silva Palácios, Terezinha Inez Estivalet Svidzinski, Paulo Cesar de Souza Pereira, Monique de Souza, Camila Barros Galinari, Renato Sonchini Gonçalves, Glaucia Sayuri Arita, Luis C. Malacarne, Karina Mayumi Sakita, Raquel Cabral Melo, Wilker Caetano, Valéria Aparecida Baquetti Mosca, Patrícia de Souza Bonfim-Mendonça, Mauro Luciano Baesso, Érika Seki Kioshima Cotica, Tiago de Paula Bianchi, Daniella Renata Faria, Gabriel Batista Cesar, Pollyanna Cristina Vincenzi Conrado |
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| Rok vydání: | 2020 |
| Předmět: |
medicine.medical_specialty
medicine.medical_treatment 030303 biophysics Biophysics Photodynamic therapy Capsules 02 engineering and technology Poloxamer Pharmacology Proinflammatory cytokine Polymerization 03 medical and health sciences chemistry.chemical_compound Mice Tinea In vivo Medicine Animals Radiology Nuclear Medicine and imaging Microsporum canis Perylene Anthracenes 0303 health sciences Radiation Photosensitizing Agents Radiological and Ultrasound Technology biology business.industry 021001 nanoscience & nanotechnology biology.organism_classification medicine.disease Hypericin Nanostructures chemistry Photochemotherapy Toxicity Histopathology Tinea capitis 0210 nano-technology business |
| Zdroj: | Journal of photochemistry and photobiology. B, Biology. 215 |
| ISSN: | 1873-2682 |
| Popis: | The antifungal application of photodynamic therapy (PDT) has been widely explored. According to superficial nature of tinea capitis and the facility of application of light sources, the use of nanoencapsulated hypericin in P-123 associated with PDT (P123-Hy-PDT) has been a poweful tool to treat this pathology. Thus, the aim of this study was to evaluate the efficiency of P123-Hy-PDT against planktonic cells and in a murine model of dermatophytosis caused by Microsporum canis. In vitro antifungal susceptibility and in vivo efficiency tests were performed, including a skin toxicity assay, analysis of clinical signs by evaluating score, and photoacoustic spectroscopy. In addition, tissue analyses by histopathology and levels of pro-inflammatory cytokines, such as quantitative and qualitative antifungal assays, were employed. The in vitro assays demonstrated antifungal susceptibility with 6.25 and 12.5 μmol/L P123-Hy-PDI; these experiments are the first that have used this treatment of animals. P123-Hyp-mediated PDT showed neither skin nor biochemical alteration in vivo; it was safe for dermatophytosis treatment. Additionally, the treatment revealed rapid improvement in clinical signs at the site of infection after only three treatment sessions, with a clinical score confirmed by photoacoustic spectroscopy. The mycological reduction occurred after six treatment sessions, with a statistically significant decrease compared with untreated infected animals. These findings showed that P123-Hy-PDT restored tissue damage caused by infection, a phenomenon confirmed by histopathological analysis and proinflammatory cytokine levels. Our results reveal for the first time that P123-Hy-PDT is a promising treatment for tinea capitis and tinea corporis caused by M. canis, because it showed rapid clinical improvement and mycological reduction without causing toxicity. |
| Databáze: | OpenAIRE |
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