The codon 399 Arg/Gln XRCC1 polymorphism is associated with lung cancer in Indians
| Autor: | Umamaheshwar Rao Naidu Madireddy, Kirmani Natukula, Usha Rani Pingali, Venkata Satya Suresh Attili, Kaiser Jamil |
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| Rok vydání: | 2013 |
| Předmět: |
Male
Cancer Research Lung Neoplasms Genotype Epidemiology DNA repair Glutamine non-small cell lung cancer (NSCLC) India Biology Adenocarcinoma Arginine Polymerase Chain Reaction XRCC1 Risk Factors Carcinoma Non-Small-Cell Lung medicine Humans Genetic Predisposition to Disease Lung cancer Codon Genotyping Neoplasm Staging Genetics Polymorphism Genetic Public Health Environmental and Occupational Health Base excision repair DNA Middle Aged medicine.disease Prognosis DNA-Binding Proteins Survival Rate X-ray Repair Cross Complementing Protein 1 Oncology Case-Control Studies Carcinoma Squamous Cell Female Restriction fragment length polymorphism Polymorphism Restriction Fragment Length Follow-Up Studies |
| Zdroj: | Asian Pacific journal of cancer prevention : APJCP. 14(9) |
| ISSN: | 2476-762X |
| Popis: | Background: The XRCC1 (X-ray repair cross complimenting group-I) gene in BER (base excision repair) pathway is essential for DNA repair process. Polymorphisms in this gene are associated with variations in the repair efficiency which might predispose individuals to development of various cancers. Two variants of XRCC1gene (at codon 399), Gln/Gln and Arg/Gln, have been shown to be related to lowered DNA repair capacity and increased genomic instability in multiple studies. Hence our investigation focused on genotyping these variants to correlate with other multiple risk factors in lung cancer (NSCLC) patients since we hypothesized that these variants of the XRCC1 gene might influence disease susceptibility. Materials and Methods: We examined the frequency of the polymorphism in one hundred cases and an almost equal number of controls after recording their demographics with a structured questionnaire. Genomic DNA from blood samples was extracted for PCR studies, followed by RFLP to determine the variants. The significance of the data was statistically analyzed. Results: The three genotypes in cases and controls were Arg/Arg (40% and 54.45%); Gln/Gln (19% and 9.90%), and Arg/Gln (41.0% and 35.64%) respectively. Among these 3 genotypes, we found Gln/Gln and Arg/Gln to show association with lung cancer. Correlating these genotypes with several parameters, we also found that these two variants were associated with risk in males (p |
| Databáze: | OpenAIRE |
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