Serodiagnosis of viral hepatitis A by a modified competitive binding radioimmunoassay for immunoglobulin M anti-hepatitis A virus
| Autor: | Daniel W. Bradley, R H Decker, James E. Maynard, Howard A. Fields, R Whittington, L R Overby, Karen A. McCaustland |
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| Rok vydání: | 1979 |
| Předmět: |
Microbiology (medical)
viruses Radioimmunoassay Antibodies Viral Immunoglobulin G Antibody Specificity medicine Animals Humans Hepatovirus Hepatitis biology fungi virus diseases Hepatitis A Convalescence biochemical phenomena metabolism and nutrition medicine.disease Virology Molecular biology digestive system diseases Immunoglobulin M Acute Disease biology.protein Antibody Viral hepatitis Protein A Research Article |
| Zdroj: | Journal of Clinical Microbiology. 9:120-127 |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.9.1.120-127.1979 |
| Popis: | A competitive binding radioimmunoassay (CBA) for antibody to hepatitis A virus (HAV) was evaluated and compared with a standard solid-phase radioimmunoassay for anti-HAV, CBA was found to be sensitive and specific for the detection of anti-HAV, as demonstrated by the 98% concordance of CBA and solid-phase radioimmunoassay test results. The standard CBA test was modified for the differential detection of acute (immunoglobulin M) and convalescent (immunoglobulin G) anti-HAV by incorporation of a step in which immunoglobulin G anti-HAV was preferentially absorbed with S. aureus cells (protein A). The modified CBA test was shown to be capable of differentiating between acute- and convalescent-phase sera. The modified CBAM test was able to detect immunoglobulin M anti-HAV up to approximately 4 weeks after the onset of illness. |
| Databáze: | OpenAIRE |
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