Production of cloned pigs from in vitro systems
| Autor: | Joellyn M. Enos, Paul J. Golueke, Kelly S. Mallon, Todd M. Forsythe, Martha Pfister-Genskow, Erik Forsberg, Steven R. Watt, Jeff Betthauser, Pavla M. Misica, Greg D. Mell, Gary R. Voelker, Lynette A. Childs, Tiffany L. Lesmeister, Kenneth J. Eilertsen, Gail L. Jurgella, Monica L. Augenstein, Michael D. Bishop, Nikolai S. Strelchenko, Simon Thompson, Marvin M. Pace, Richard W. Koppang |
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| Rok vydání: | 2000 |
| Předmět: |
Male
Swine Somatic cell Cloning Organism Xenotransplantation medicine.medical_treatment Transplantation Heterologous Biomedical Engineering Cell Count Bioengineering Fertilization in Vitro Biology Transfection Applied Microbiology and Biotechnology Andrology Fetus Pregnancy Culture Techniques medicine Animals Humans Cells Cultured Cell Nucleus Cloning Genetic transfer Reproducibility of Results Cell Differentiation Embryo culture DNA Embryo Transfer Embryo transfer In vitro maturation Cell culture Immunology Oocytes Molecular Medicine Female Microsatellite Repeats Biotechnology |
| Zdroj: | Nature Biotechnology. 18:1055-1059 |
| ISSN: | 1546-1696 1087-0156 |
| DOI: | 10.1038/80242 |
| Popis: | Here we describe a procedure for cloning pigs by the use of in vitro culture systems. Four healthy male piglets from two litters were born following nuclear transfer of cultured somatic cells and subsequent embryo transfer. The initiation of five additional pregnancies demonstrates the reproducibility of this procedure. Its important features include extended in vitro culture of fetal cells preceding nuclear transfer, as well as in vitro maturation and activation of oocytes and in vitro embryo culture. The cell culture and nuclear transfer techniques described here should allow the use of genetic modification procedures to produce tissues and organs from cloned pigs with reduced immunogenicity for use in xenotransplantation. |
| Databáze: | OpenAIRE |
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