Combined autofluorescence and diffuse reflectance for brain tumour surgical guidance: initial ex vivo study results
| Autor: | Kevin Manley, Kevin Buckley, Gerard Nunan, Michael Jansen, Konstantin Grygoryev, Stefan Andersson-Engels, Michael O'Sullivan, Huihui Lu, Ray Burke, Niamh Bermingham |
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| Rok vydání: | 2021 |
| Předmět: |
0303 health sciences
Diffuse reflectance infrared fourier transform Chemistry Optical measurements Grey matter Linear discriminant analysis 01 natural sciences Article Atomic and Molecular Physics and Optics Cross-validation 010309 optics 03 medical and health sciences Autofluorescence medicine.anatomical_structure 0103 physical sciences medicine Diffuse reflection Ex vivo 030304 developmental biology Biotechnology Biomedical engineering |
| Zdroj: | Biomed Opt Express |
| ISSN: | 2156-7085 |
| DOI: | 10.1364/boe.420292 |
| Popis: | This ex vivo study was conducted to assess the potential of using a fibre optic probe system based on autofluorescence and diffuse reflectance for tissue differentiation in the brain. A total of 180 optical measurements were acquired from 28 brain specimens (five patients) with eight excitation and emission wavelengths spanning from 300 to 700 nm. Partial least square-linear discriminant analysis (PLS-LDA) was used for tissue discrimination. Leave-one-out cross validation (LOOCV) was then used to evaluate the performance of the classification model. Grey matter was differentiated from tumour tissue with sensitivity of 89.3% and specificity of 92.5%. The variable importance in projection (VIP) derived from the PLS regression was applied to wavelengths selection, and identified the biochemical sources of the detected signals. The initial results of the study were promising and point the way towards a cost-effective, miniaturized hand-held probe for real time and label-free surgical guidance. |
| Databáze: | OpenAIRE |
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