Response Surface Methodology to Optimize the Isolation of Dominant Volatile Compounds from Monofloral Greek Thyme Honey Using SPME-GC-MS

Autor:	Christos S. Pappas, Panagiota-Kyriaki Revelou, Alexandra Skouria, Petros A. Tarantilis, Marinos Xagoraris, Eleftherios Alissandrakis
Rok vydání:	2021
Předmět:	Pharmaceutical Science Fraction (chemistry) Spme gc ms thyme honey Solid-phase microextraction 01 natural sciences Gas Chromatography-Mass Spectrometry Article Analytical Chemistry Thymus Plant response surface methodology Matrix (chemical analysis) QD241-441 0404 agricultural biotechnology Drug Discovery Response surface methodology Physical and Theoretical Chemistry Solid Phase Microextraction Volatile Organic Compounds Chromatography Greece Chemistry 010401 analytical chemistry Organic Chemistry Extraction (chemistry) Honey 04 agricultural and veterinary sciences 040401 food science 0104 chemical sciences volatiles Chemistry (miscellaneous) solid-phase microextraction Multiple response optimization Molecular Medicine Gas chromatography–mass spectrometry optimization
Zdroj:	Molecules Volume 26 Issue 12 Molecules, Vol 26, Iss 3612, p 3612 (2021)
ISSN:	1420-3049
DOI:	10.3390/molecules26123612
Popis:	This study aimed at an experimental design of response surface methodology (RSM) in the optimization of the dominant volatile fraction of Greek thyme honey using solid-phase microextraction (SPME) and analyzed by gas chromatography-mass spectrometry (GC-MS). For this purpose, a multiple response optimization was employed using desirability functions, which demand a search for optimal conditions for a set of responses simultaneously. A test set of eighty thyme honey samples were analyzed under the optimum conditions for validation of the proposed model. The optimized combination of isolation conditions was the temperature (60 °C), equilibration time (15 min), extraction time (30 min), magnetic stirrer speed (700 rpm), sample volume (6 mL), water: honey ratio (1:3 v/w) with total desirability over 0.50. It was found that the magnetic stirrer speed, which has not been evaluated before, had a positive effect, especially in combination with other factors. The above-developed methodology proved to be effective in the optimization of isolation of specific volatile compounds from a difficult matrix, like honey. This study could be a good basis for the development of novel RSM for other monofloral honey samples.
Databáze:	OpenAIRE
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