NOVEL ANALYTICAL STUDY FOR REACTION INTERMEDIATES IN THE PRIMARY RADIATION INTERACTION OF DNA USING A SYNCHROTRON RADIATION-INDUCED LUMINESCENCE SPECTROSCOPY
Autor: | Y Kodashima, C. Küstner-Wetekam, D Bloβ, Xaver Holzapfel, H Makishima, Akinari Yokoya, S Takada, Arno Ehresmann, Yuji Saitoh, Ph. Schmidt, H Aihara, Masatoshi Ukai, H. Otto, Hiroyuki Shimada, S Nakiri, Yoshihiro Fukuda, Christian Ozga, Kentaro Fujii, T Kojima, André Knie |
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Rok vydání: | 2018 |
Předmět: |
Materials science
Luminescence Nitrogen Deoxyribonucleotides Quantum yield Synchrotron radiation Reaction intermediate Photochemistry 030218 nuclear medicine & medical imaging 03 medical and health sciences 0302 clinical medicine Elementary reaction Radiology Nuclear Medicine and imaging Spectroscopy Absorption (electromagnetic radiation) Radiation Radiological and Ultrasound Technology Public Health Environmental and Occupational Health Water General Medicine DNA Equipment Design Hydrogen-Ion Concentration XANES Oxygen X-Ray Absorption Spectroscopy 030220 oncology & carcinogenesis Synchrotrons |
Zdroj: | Radiation protection dosimetry. 183(1-2) |
ISSN: | 1742-3406 |
Popis: | To identify the precise molecular processes to induce DNA lesions, we attempt a novel spectroscopy of X-ray induced luminescence (XIL) using soft X-ray synchrotron radiation, which is a non-destructive analysis of the reaction intermediates in the elementary reaction pathway of damage induction and self-organized restoration. Using a liquid micro-jet technique to introduce aqueous samples in a vacuum chamber, we measure UV-visible luminescence from nucleotide solution as a function of the soft X-ray energy from the nitrogen to oxygen K-edge region. The XIL intensities for the nucleotide solutions are significantly enhanced in the soft X-ray region (410-530 eV) which is ascribed to the K-shell excitation/ionization of nitrogen atoms in the nucleobases. Furthermore, the XIL spectra do not show any signature of X-ray absorption near-edge structure (XANES) of the nucleobases. This is because the luminescence intensities collected from the integral area of the micro-jet only reflect the quantum yield of luminescence of the absorbed X-ray into UV-visible light irrespective of the absorption cross sections, i.e. of XANES. Thus the present result is the first evidence of luminescence as a result of X-ray absorption of aqueous nucleotides. |
Databáze: | OpenAIRE |
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