Molecular Phylogenetics and Population Genetics of the Dengue VectorAedes aegyptiFrom the Arabian Peninsula
| Autor: | Emad I.M. Khater, Hany A. Kamal, Amgad A. Saleh, Jeffery R. Powell, Farrukh Baig |
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| Rok vydání: | 2021 |
| Předmět: |
030231 tropical medicine
Population Saudi Arabia Zoology Population genetics Aedes aegypti 03 medical and health sciences 0302 clinical medicine Aedes Genetic variation Animals education Phylogeny 030304 developmental biology 0303 health sciences education.field_of_study General Veterinary Phylogenetic tree biology fungi Genetic Variation biology.organism_classification Infectious Diseases Genetic marker Insect Science Vector (epidemiology) Molecular phylogenetics Parasitology |
| Zdroj: | Journal of Medical Entomology. 58:2161-2176 |
| ISSN: | 1938-2928 0022-2585 |
| Popis: | Aedes aegypti mosquito is the principal dengue vector in the Kingdom of Saudi Arabia (KSA); however, no study has addressed its ecology and population structure yet. Therefore, we report on Ae. aegypti phylo- and population genetics using three DNA markers: COI, ND4, and rDNA-ITS2. Sampling the immature stages of Ae. aegypti revealed that water storage tanks (34.3% of habitats) were the most productive and contained 33% of immatures stages. Other important habitats included containers for wastewater drainage (including air-conditioning and water cooler trays) and containers associated with ornamentation. Shallow water leakage spots (2.7% of habitats, 8% of immatures) can be considered rare-but-epidemiologically-important containers. Neighbor-joining (NJ) phylogenetic analysis of Ae. aegypti identified 8, 14, and 9 haplotypes of COI, ND4, and ITS2, respectively, and revealed high levels of genetic variation in Ae. aegypti populations of KSA. Global distribution of haplotypes also indicated multiple gene introductions into these populations, with high levels of intra-population genetic variation and continuous gene exchange. The neutrality values indicated a deficiency of alleles and suggested that the KSA Ae. aegypti loci tested did not follow a neutral model of molecular evolution. Fst values and AMOVA indicated that most of the genetic variation in the KSA Ae. aegypti populations is due to intra- rather than inter-population differences. This is the first comprehensive report on the phylo- and population genetics of Ae. aegypti from the Arabian Peninsula. This information expands our understanding of the ecology and population dynamics of this important arboviral vector for informed control efforts. |
| Databáze: | OpenAIRE |
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