Characterization of three-dimensional bone-like tissue growth and organization under influence of directional fluid flow
Autor: | Bregje W. M. de Wildt, Feihu Zhao, Iris Lauwers, Bert van Rietbergen, Keita Ito, Sandra Hofmann |
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Přispěvatelé: | Bioengineering Bone, Orthopaedic Biomechanics, Biomedical Engineering, Eindhoven MedTech Innovation Center, ICMS Core |
Jazyk: | angličtina |
Rok vydání: | 2023 |
Předmět: |
Cultured
Bone Regeneration Tissue Scaffolds extracellular matrix Cells Bioengineering Mesenchymal Stem Cells Cell Differentiation Applied Microbiology and Biotechnology Bone and Bones Osteogenesis in vitro model computational fluid dynamics model Tissue Engineering/methods Humans cell and tissue organization bone tissue engineering Cells Cultured Biotechnology |
Zdroj: | Biotechnology and Bioengineering, 120(7), 2013-2026. Wiley-VCH Verlag |
ISSN: | 0006-3592 |
Popis: | The transition in the field of bone tissue engineering from bone regeneration to in vitro models has come with the challenge of recreating a dense and anisotropic bone-like extracellular matrix (ECM). Although the mechanism by which bone ECM gains its structure is not fully understood, mechanical loading and curvature have been identified as potential contributors. Here, guided by computational simulations, we evaluated cell and bone-like tissue growth and organization in a concave channel with and without directional fluid flow stimulation. Human mesenchymal stromal cells were seeded on donut-shaped silk fibroin scaffolds and osteogenically stimulated for 42 days statically or in a flow perfusion bioreactor. After 14, 28, and 42 days, constructs were investigated for cell and tissue growth and organization. As a result, directional fluid flow was able to improve organic tissue growth but not organization. Cells tended to orient in the tangential direction of the channel, possibly attributed to its curvature. Based on our results, we suggest that organic ECM production but not anisotropy can be stimulated through the application of fluid flow. With this study, an initial attempt in three-dimensions was made to improve the resemblance of in vitro produced bone-like ECM to the physiological bone ECM. |
Databáze: | OpenAIRE |
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