Zinspy Sensors with Enhanced Dynamic Range for Imaging Neuronal Cell Zinc Uptake and Mobilization
| Autor: | Rodney P. Feazell, Jubin W. Ryu, Stephen J. Lippard, Jacek Jaworski, Elizabeth M. Nolan, Morgan Sheng |
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| Rok vydání: | 2006 |
| Předmět: |
Cell Survival
Stereochemistry Glutamic Acid chemistry.chemical_element Biosensing Techniques Zinc Crystallography X-Ray Hippocampus Biochemistry Article Catalysis Rats Sprague-Dawley Metal chemistry.chemical_compound Colloid and Surface Chemistry Chlorocebus aethiops Thiophene Animals Humans Benzoic acid Neurons Microscopy Confocal Molecular Structure Halogenation General Chemistry Fluoresceins Fluorescence Rats Dissociation constant Spectrometry Fluorescence chemistry visual_art COS Cells Dentate Gyrus visual_art.visual_art_medium Thermodynamics Selectivity HeLa Cells Nuclear chemistry |
| Zdroj: | Journal of the American Chemical Society. 128:15517-15528 |
| ISSN: | 1520-5126 0002-7863 |
| DOI: | 10.1021/ja065759a |
| Popis: | Thiophene moieties were incorporated into previously described Zinspy (ZS) fluorescent Zn(II) sensor motifs (Nolan, E. M.; Lippard, S. J. Inorg. Chem. 2004, 43, 8310–8317) to provide enhanced fluorescence properties, low-micromolar dissociation constants for Zn(II), and improved Zn(II) selectivity. Halogenation of the xanthenone and benzoate moieties of the fluorescein platform systematically modulates the excitation and emission profiles, pH-dependent fluorescence, Zn(II) affinity, and Zn(II) complexation rates, offering a general strategy for tuning multiple properties of xanthenone-based metal ion sensors. Extensive biological studies in cultured cells and primary neuronal cultures demonstrate 2-{6-hydroxy-3-oxo-4,5-bis[(pyridin-2-ylmethylthiophen-2-ylmethylamino)methyl]-3H-xanthen-9-yl}benzoic acid (ZS5) to be a versatile imaging tool for detecting Zn(II) in vivo. ZS5 localizes to the mitochondria of HeLa cells and allows visualization of glutamate-mediated Zn(II) uptake in dendrites and Zn(II) release resulting from nitrosative stress in neurons. |
| Databáze: | OpenAIRE |
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