Role of syndecan-1 and exogenous heparin in hepatoma sphere formation
| Autor: | TingTing Tseng, Shih-Chiang Lin, Yaoyun Jhang, ShaoChen Lee, Ching-Po Wu |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Carcinoma
Hepatocellular Cellular differentiation Cell CD13 Antigens Disaccharides Biochemistry Polymerase Chain Reaction Syndecan 1 03 medical and health sciences 0302 clinical medicine Cancer stem cell Cell Line Tumor Spheroids Cellular Gene expression medicine Biomarkers Tumor Humans Molecular Biology 030304 developmental biology 0303 health sciences biology Chemistry Heparin CD44 Liver Neoplasms Cell Biology Hep G2 Cells Cell biology Up-Regulation Gene Expression Regulation Neoplastic medicine.anatomical_structure Hyaluronan Receptors Proteoglycan 030220 oncology & carcinogenesis biology.protein Neoplastic Stem Cells Proteoglycans Syndecan-1 Stem cell Chondroitin |
| Zdroj: | Biochemistry and cell biology = Biochimie et biologie cellulaire. 98(2) |
| ISSN: | 1208-6002 |
| Popis: | Glycosaminoglycan-modified proteoglycans play important roles in many cell activities, including cell differentiation and stem cell development. Tumor sphere formation ability is one of properties in cancer stem cells (CSCs). The correlation between CSC markers and proteoglycan remains to be clarified. Upon hepatoma sphere formation, expression of CSC markers CD13, CD90, CD133, and CD44, as well the syndecan family protein syndecan-1 (SDC1), increased as analyzed by PCR. Further examination by suppression of CD13 expression showed downregulation of SDC1 and CD44 gene expression, whereas suppression of SDC1 gene expression downregulated CD13 and CD44 gene expression. Suppression of SDC1 gene expression also suppressed sphere development, as analyzed by a novel sphereocrit assay to quantify the level of sphere formation. The heparin disaccharide components, but not those of chondroitin disaccharide, changed with hepatoma sphere development, revealing the increased levels of N-sulfation and 2-O-sulfation. These explained the inhibition of hepatoma sphere formation by exogenous heparin. In conclusion, we found that SDC1 affected CSC marker CD13 and CD44 expression. SDC1 proteoglycan and heparin components changed and affected hepatoma sphere development. Application of heparin mimics in reduction of hepatoma stem cells might be possible. |
| Databáze: | OpenAIRE |
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