Differential Regulation of Growth and Checkpoint Control Mediated by a Cdc25 Mitotic Phosphatase from Pneumocystis carinii
| Autor: | Charles F. Thomas, Michael P. Gustafson, Edward B. Leof, Andrew H. Limper, Frank Rusnak |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
DNA Replication
Cdc25 Molecular Sequence Data Mitosis Biology Biochemistry Fungal Proteins Saccharomyces Gene Expression Regulation Fungal parasitic diseases Animals cdc25 Phosphatases Amino Acid Sequence RNA Messenger Cloning Molecular Molecular Biology Gene Pneumocystis Cell Cycle Genetic Complementation Test Temperature RNA Fungal Cell Biology G2-M DNA damage checkpoint Cell cycle bacterial infections and mycoses biology.organism_classification Virology Rats respiratory tract diseases Cell biology DNA replication checkpoint Kinetics enzymes and coenzymes (carbohydrates) Pneumocystis carinii Mutation Schizosaccharomyces pombe biology.protein biological phenomena cell phenomena and immunity Sequence Alignment DNA Damage |
| Zdroj: | Journal of Biological Chemistry. 276:835-843 |
| ISSN: | 0021-9258 |
| DOI: | 10.1074/jbc.m007814200 |
| Popis: | Pneumocystis carinii is an opportunistic fungal pathogen phylogenetically related to the fission yeast Schizosaccharomyces pombe. P. carinii causes severe pneumonia in immunocompromised patients with AIDS and malignancies. Although the life cycle of P. carinii remains poorly characterized, morphologic studies of infected lung tissue indicate that P. carinii alternates between numerous small trophic forms and fewer large cystic forms. To understand further the molecular mechanisms that regulate progression of the cell cycle of P. carinii, we have sought to identify and characterize genes in P. carinii that are important regulators of eukaryotic cell cycle progression. In this study, we have isolated a cDNA from P. carinii that exhibits significant homology, but unique functional characteristics, to the mitotic phosphatase Cdc25 found in S. pombe. P. carinii Cdc25 was shown to rescue growth of the temperature-sensitive S. pombe cdc25-22 strain and thus provides an additional tool to investigate the unique P. carinii life cycle. Although P. carinii Cdc25 could also restore the DNA damage checkpoint in cdc25-22 cells, it was unable to restore fully the DNA replication checkpoint. The dissociation of checkpoint control at the level of Cdc25 indicates that Cdc25 may be under distinct regulatory control in mediating checkpoint signaling. |
| Databáze: | OpenAIRE |
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