Fabrication of immobilized enzyme reactors with pillar arrays into polydimethylsiloxane microchip
| Autor: | Attila Gáspár, Cynthia Nagy, Adam Kecskemeti |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Fabrication
Immobilized enzyme Swine Protein digestion Microfluidics Serum Albumin Human 02 engineering and technology 01 natural sciences Biochemistry Soft lithography Analytical Chemistry chemistry.chemical_compound Digestion (alchemy) Lab-On-A-Chip Devices Viperidae Animals Humans Environmental Chemistry Trypsin Dimethylpolysiloxanes Spectroscopy Polydimethylsiloxane Chemistry 010401 analytical chemistry Enzymes Immobilized 021001 nanoscience & nanotechnology 0104 chemical sciences Volumetric flow rate Chemical engineering Proteolysis 0210 nano-technology Snake Venoms |
| Zdroj: | Analytica Chimica Acta. 1108:70-78 |
| ISSN: | 0003-2670 |
| DOI: | 10.1016/j.aca.2020.02.048 |
| Popis: | This paper demonstrates the design, efficiency and applicability of a simple and inexpensive microfluidic immobilized enzymatic reactor (IMER) for rapid protein digestion. The high surface-to-volume ratio (S/V) of the reactor was achieved by forming pillars in the channel. It was found that pillar arrays including dimensions of 40 μm × 40 μm as pillar diameter and interpillar distance can provide both relatively high S/V and flow rate in the PDMS chip, the fabrication of which was performed by means of soft lithography using average research laboratory infrastructure. CZE peptide maps of IMER-based digestions were compared to peptide maps obtained from standard in-solution digestion of proteins. The peak patterns of the electropherograms and the identified proteins were similar, however, digestion with the IMER requires less than 10 min, while in-solution digestion takes 16 h. |
| Databáze: | OpenAIRE |
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