Evaluation of the Morphology and Biocompatibility of Natural Silk Fibers/Agar Blend Scaffolds for Tissue Regeneration
| Autor: | Vo Van Thanh Niem, Vo Van Toi, Pham Dinh Chuong, Luong Thu-Hien, Bui Chi Bao, Nguyen Thi Hiep, Huynh Chan Khon, Nguyen Thanh-Truc, Nguyen Dai Hai, Mai Ngoc Tuan Anh |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Materials science
Article Subject Polymers and Plastics Eosin biology Biocompatibility Scanning electron microscope Chemical structure 02 engineering and technology Haematoxylin 010402 general chemistry 021001 nanoscience & nanotechnology biology.organism_classification lcsh:Chemical technology 01 natural sciences 0104 chemical sciences Staining chemistry.chemical_compound SILK chemistry Bombyx mori lcsh:TP1-1185 0210 nano-technology Biomedical engineering |
| Zdroj: | International Journal of Polymer Science, Vol 2018 (2018) |
| ISSN: | 1687-9430 1687-9422 |
| Popis: | This study was aimed to develop a tissue engineering scaffold by incorporation of Bombyx mori silk fiber (BMSF) and agar. This promised the improvement in enhancing their advantageous properties as well as limiting their defects without occurring chemical reactions or crosslink formation. The morphology and chemical structure of scaffolds were observed using scanning electron microscope (SEM) observation and Fourier transform infrared (FT-IR) spectra. The SEM results show that scaffolds containing BMSF have microporous structures, which are suitable for cell adhesion. Agar scaffolds, by contrast, had much more flat morphology. FT-IR spectra confirm that no modifications to BMSF happened in scaffolds, which indicates that there was no chemical reaction or crosslink formation between silk and agar in this process. Furthermore, the biocompatibility of scaffolds was performed in the mouse’s subcutaneous part of the dorsal region for 15 days, followed by Haematoxylin and Eosin (H&E) staining. H&E staining results demonstrate that scaffolds had good biocompatibility and there was no sign of the body rejection in all of samples. The results from animal study show that SA scaffolds have the most stable structure for cell adhesion compared with those single materials. |
| Databáze: | OpenAIRE |
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