MALDI-ToF protein profiling as a potential rapid diagnostic platform for COVID-19

Autor: Pratool Bharti, Prajkta Chivte, Elizabeth R. Gaillard, Venkata Devesh Reddy Seethi, Shrihari S. Kadkol, Zane LaCasse, Joshua Bland
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Saliva
Diagnostic methods
Coronavirus disease 2019 (COVID-19)
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
Concordance
Clinical Biochemistry
IgG
immunoglobulin G
N Protein
nucleocapsid protein
Immunoglobulins
MALDI-ToF MS
matrix-assisted laser desorption/ionization-time of flight mass spectrometry
COVID-19 testing
ACE2
angiotensin-converting enzyme 2
IgA
immunoglobulin A
Microbiology
S Protein
spike protein
SARS-CoV-2
severe acute respiratory syndrome coronavirus 2
M Protein
membrane protein
AUC
area under the curve
RBD
receptor binding domain
EUA
emergency use authorization
Medical technology
Medicine
R855-855.5
Spectroscopy
COVID-19
coronavirus disease 2019
MALDI-ToF
LoD
limit of detection
LC-MS
liquid chromatography mass spectrometry
business.industry
Ct
cycle threshold
DTT
dithiothreitol
VEP
viral envelope protein
Asymptomatic
Protein profiling
Medical Laboratory Technology
IgM
immunoglobulin M
FDA
food and drug administration
Potential biomarkers
Proteome
Immunology
RNA
ribonucleic acid
E Protein
envelope protein
WHO
world health organization
ROC
receiver operating characteristic
RT-qPCR
reverse transcriptase quantitative polymerase chain reaction
business
NP
nasopharyngeal
Research Article
Zdroj: Journal of Mass Spectrometry and Advances in the Clinical Lab
Journal of Mass Spectrometry and Advances in the Clinical Lab, Vol 21, Iss, Pp 31-41 (2021)
ISSN: 2667-145X
2667-1468
Popis: More than a year after the COVID-19 pandemic was declared, the need still exists for accurate, rapid, inexpensive and non-invasive diagnostic methods that yield high specificity and sensitivity towards the current and newly emerging SARS-CoV-2 strains. Compared to the nasopharyngeal swabs, several studies have established saliva as a more amenable specimen type for early detection of SARS-CoV-2. Considering the limitations and high demand for COVID-19 testing, we employed MALDI-ToF mass spectrometry in the analysis of 60 gargle samples from human donors and compared the resultant spectra against COVID-19 status. Several standards, including isolated human serum immunoglobulins, and controls, such as pre-COVID-19 saliva and heat inactivated SARS-CoV-2 virus, were simultaneously analyzed to provide a relative view of the saliva and viral proteome as they would appear in this workflow. Five potential biomarker peaks were established that demonstrated high concordance with COVID-19 positive individuals. Overall, the agreement of these results with RT-qPCR testing on nasopharyngeal swabs was ≥90% for the studied cohort, which consisted of young and largely asymptomatic student athletes. From a clinical standpoint, the results from this pilot study suggest that MALDI-ToF could be used to develop a relatively rapid and inexpensive COVID-19 assay.
Databáze: OpenAIRE
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