The intracellular domain of interferon-alpha receptor 2c (IFN-alphaR2c) chain is responsible for Stat activation
| Autor: | Carolyn Lee, Serguei V. Kotenko, Sidney Pestka, Olga V. Mirochnitchenko, Lara S. Izotova |
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| Rok vydání: | 1999 |
| Předmět: |
STAT3 Transcription Factor
Recombinant Fusion Proteins Receptor Interferon alpha-beta Hybrid Cells Cell Line Cricetinae Animals Humans STAT1 STAT2 Receptor STAT3 STAT4 Receptors Interferon Multidisciplinary biology Interferon-alpha Biological Sciences Glycoprotein 130 Molecular biology DNA-Binding Proteins STAT1 Transcription Factor Gene Expression Regulation biology.protein Trans-Activators Signal transduction Intracellular Signal Transduction |
| Zdroj: | Proceedings of the National Academy of Sciences of the United States of America. 96(9) |
| ISSN: | 0027-8424 |
| Popis: | Type I IFNs activate the Jak-Stat signal transduction pathway. The IFN-alpha receptor 1 (IFN-alphaR1) subunit and two splice variants of the IFN-alphaR2 subunit, IFN-alphaR2c and IFN-alphaR2b, are involved in ligand binding. All these receptors have been implicated in cytokine signaling and, specifically, in Stat recruitment. To evaluate the specific contribution of each receptor subunit to Stat recruitment we employed chimeric receptors with the extracellular domain of either IFN-gammaR2 or IFN-gammaR1 fused to the intracellular domains of IFN-alphaR1, IFN-alphaR2b, and IFN-alphaR2c. These chimeric receptors were expressed in hamster cells. Because human IFN-gamma exhibits no activity on hamster cells, the use of the human IFN-gamma receptor extracellular domains allowed us to avoid the variable cross-species activity of the type I IFNs and eliminate the possibility of contributions of endogenous type I IFN receptors into the Stat recruitment process. We demonstrate that Stat recruitment is solely a function of the IFN-alphaR2c intracellular domain. When chimeric receptors with the human IFN-gammaR1 extracellular domain and various human IFN-alpha receptor intracellular domains were expressed in hamster cells carrying the human IFN-gammaR2 subunit, only the IFN-alphaR2c subunit was capable of supporting IFN-gamma signaling as measured by MHC class I induction, antiviral protection, and Stat activation. Neither the IFN-alphaR2b nor the IFN-alphaR1 intracellular domain was able to recruit Stats or support IFN-gamma-induced biological activities. Thus, the IFN-alphaR2c intracellular domain is necessary and sufficient to activate Stat1, Stat2, and Stat3 proteins. |
| Databáze: | OpenAIRE |
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