A Microtus fortisprotein, serum albumin, is a novel inhibitor of Schistosoma japonicumschistosomula
| Autor: | Qiang Gong, Guo-Jun Wu, Wei-Xin Hu, De-Hui Xiong, Ruan-Jing Yu, Rong Li |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Microbiology (medical)
lcsh:Arctic medicine. Tropical medicine lcsh:RC955-962 serum albumin Serum albumin lcsh:QR1-502 Schistosomiasis Microtus fortis Chromatography Affinity lcsh:Microbiology Schistosoma japonicum Andrology parasitic diseases medicine Animals Incubation Eggs per gram biology Arvicolinae Albumin Articles medicine.disease biology.organism_classification Praziquantel Immunology biology.protein Digestion medicine.drug |
| Zdroj: | Memórias do Instituto Oswaldo Cruz, Volume: 108, Issue: 7, Pages: 865-872, Published: NOV 2013 Memórias do Instituto Oswaldo Cruz., Vol 108, Iss 7, Pp 865-872 (2013) Memórias do Instituto Oswaldo Cruz |
| Popis: | Schistosomiasis is an endemic parasite disease and praziquantel is the only drug currently in use to control this disease. Experimental and epidemiological evidence strongly suggests that Microtus fortis ( Mf ) is a naturally resistant vertebrate host of Schistosoma japonicum . In the present study, we found that Mf serum albumin ( Mf -albumin) and the conditioned medium of pcDNA3.1- Mf -albumin caused 46.2% and 38.7% schistosomula death rates in 96 h, respectively, which were significantly higher than that of the negative control (p < 0.05). We also found that mice injected with Mf -albumin had a 43.5% reduction in worm burden and a 48.1% reduction in liver eggs per gram (p < 0.05) in comparison to the control animals. To characterise the mechanisms involved in clearance, schistosomula were incubated with fluorescein isothiocyanate-labelled Mf -albumin and fluorescent enrichment effects were found in the gut lumen of schistosomula after 48 h of incubation. Next, digestive tract excretions from schistosomula were collected and the sensitivity of Mf -albumin to digestive tract excretions was evaluated. The results indicated that schistosomula digestive tract excretions showed indigestibility of Mf -albumin. The death of schistosomula could be partially attributed to the lack of digestion of Mf -albumin by digestive tract excretions during the development of the schistosomula stage. Therefore, these data indicate the potential of Mf -albumin as one of the major selective forces for schistosomiasis. |
| Databáze: | OpenAIRE |
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