Cross-Reaction or Co-Infection? Serological Discrimination of Antibodies Directed against Dugbe and Crimean-Congo Hemorrhagic Fever Orthonairovirus in Nigerian Cattle

Autor:	Markus Keller, O. B. Daodu, James O. Olopade, Julia Hartlaub, Martin H. Groschup, Daniel Oladimeji Oluwayelu, Balal Sadeghi
Rok vydání:	2021
Předmět:	0301 basic medicine cross-reactivity 030231 tropical medicine Cattle Diseases Nigeria serology specificity Enzyme-Linked Immunosorbent Assay Crimean-Congo hemorrhagic fever orthonairovirus Cross Reactions Biology Antibodies Viral Immunofluorescence medicine.disease_cause Cross-reactivity Microbiology Neutralization Article Serology 03 medical and health sciences Ticks 0302 clinical medicine Seroepidemiologic Studies Virology medicine Animals Seroprevalence Nairobi sheep disease virus medicine.diagnostic_test Coinfection Dugbe orthonairovirus Ruminants sensitivity QR1-502 3. Good health 030104 developmental biology Infectious Diseases CCHFV Tick-Borne Diseases cattle Immunoglobulin G Hemorrhagic Fever Virus Crimean-Congo biology.protein Antibody DUGV Co infection
Zdroj:	Viruses Viruses, Vol 13, Iss 1398, p 1398 (2021) Volume 13 Issue 7
ISSN:	1999-4915
DOI:	10.3390/v13071398
Popis:	Dugbe orthonairovirus (DUGV) and Crimean-Congo hemorrhagic fever orthonairovirus (CCHFV) are tick-borne arboviruses within the order Bunyavirales. Both viruses are endemic in several African countries and can induce mild (DUGV, BSL 3) or fatal (CCHFV, BSL 4) disease in humans. Ruminants play a major role in their natural transmission cycle. Therefore, they are considered as suitable indicator animals for serological monitoring studies to assess the risk for human infections. Although both viruses do not actually belong to the same serogroup, cross-reactivities have already been reported earlier—hence, the correct serological discrimination of DUGV and CCHFV antibodies is crucial. In this study, 300 Nigerian cattle sera (150 CCHFV seropositive and seronegative samples, respectively) were screened for DUGV antibodies via N protein-based ELISA, indirect immunofluorescence (iIFA) and neutralization assays. Whereas no correlation between the CCHFV antibody status and DUGV seroprevalence data could be demonstrated with a newly established DUGV ELISA, significant cross-reactivities were observed in an immunofluorescence assay. Moreover, DUGV seropositive samples did also cross-react in a species-adapted commercial CCHFV iIFA. Therefore, ELISAs seem to be able to reliably differentiate between DUGV and CCHFV antibodies and should preferentially be used for monitoring studies. Positive iIFA results should always be confirmed by ELISAs.
Databáze:	OpenAIRE
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