Monitoring of bacterial community structure and growth: An alternative tool for biofilm microanalysis
| Autor: | Daniella da Costa Pereira, Simone Pennafirme, Mirian Araújo Carlos Crapez, Guilherme O.A. da Silva, Inayá Lima, Carolina Coelho da Costa Waite, Ricardo Tadeu Lopes |
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| Rok vydání: | 2020 |
| Předmět: |
lcsh:Biotechnology
Microorganism lcsh:QR1-502 engineering.material Applied Microbiology and Biotechnology Microbiology Microanalysis lcsh:Microbiology Article Extracellular polymeric substance Bioremediation lcsh:TP248.13-248.65 Molecular Biology Biofilm growth Pollutant Bacteria Chemistry Biofilm Environmental monitoring Cell Biology biochemical phenomena metabolism and nutrition Microtomography engineering Biochemical engineering Biopolymer |
| Zdroj: | Biofilm Biofilm, Vol 2, Iss, Pp 100034-(2020) |
| ISSN: | 2590-2075 |
| Popis: | Microorganisms, such as bacteria, tend to aggregate and grow on surfaces, secreting extracellular polymeric substances (EPS), forming biofilms. Biofilm formation is a life strategy, because through it microorganisms can create their own microhabitats. Whether for remediation of pollutants or application in the biomedical field, several methodological approaches are necessary for a more accurate analysis of the role and potential use of bacterial biofilms. The use of computerized microtomography to monitor biofilm growth appears to be an advantageous tool due to its non-destructive character and its ability to render 2D and 3D visualization of the samples. In this study, we used several techniques such as analysis of microbiological parameters and biopolymer concentrations to corroborate porosity quantified by 2D and 3D imaging. Quantification of the porosity of samples by microtomography was verified by increased enzymatic activity and, consequently, higher EPS biopolymer synthesis to form biofilm, indicating growth of the biofilm over 96 h. Our interdisciplinary approach provides a better understanding of biofilm growth, enabling integrated use of these techniques as an important tool in bioremediation studies of environments impacted by pollutants. Highlights • Biofilm formation is a strategy by which bacteria can create their own microhabitats. • Interdisciplinary approach provides a better understanding of biofilm growth. • Integrated use of techniques is an important tool in bioremediation studies. • X-ray microtomography proved to be a viable technique for monitoring biofilm growth. |
| Databáze: | OpenAIRE |
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