Sciatic nerve regeneration after traumatic injury using magnetic targeted adipose-derived mesenchymal stem cells
| Autor: | Vanina Usach, Clara P. Setton-Avruj, D. F. Coral, Marianela Vence, Paula A. Soto, Anna Roig, Gonzalo Piñero, Alicia Cueto, Marcela Beatriz Fernández van Raap, Diego Muraca |
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| Přispěvatelé: | Consejo Nacional de Investigaciones Científicas y Técnicas (Argentina), Agencia Nacional de Promoción Científica y Tecnológica (Argentina), Universidad Nacional de La Plata, Universidad de Buenos Aires |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Wallerian degeneration
0206 medical engineering Biomedical Engineering 02 engineering and technology Stem cells Sciatic nerve Biochemistry Biomaterials Cell therapy Magnetic targeting Peripheral Nerve Injuries Humans Medicine Remyelination Molecular Biology business.industry Magnetic Phenomena Regeneration (biology) Mesenchymal stem cell Mesenchymal Stem Cells General Medicine 021001 nanoscience & nanotechnology medicine.disease 020601 biomedical engineering Nerve Regeneration medicine.anatomical_structure Nanomedicine Stem cell 0210 nano-technology business Neuroscience Biotechnology Adult stem cell |
| Zdroj: | Digital.CSIC: Repositorio Institucional del CSIC Consejo Superior de Investigaciones Científicas (CSIC) Digital.CSIC. Repositorio Institucional del CSIC instname |
| Popis: | Traumatic peripheral nerve injuries constitute a huge concern to public health. Nerve damage leads to a decrease or even loss of mobility of the innervated area. Adult stem cell therapies have shown some encouraging results and have been identified as promising treatment candidates for nerve regeneration. A major obstacle to that approach is securing a sufficient number of cells at the injured site to produce measurable therapeutic effects. The present work tackles this issue and demonstrates enhanced nerve regeneration ability promoted by magnetic targeted cell therapy in an in vivo Wallerian degeneration model. To this end, adipose-derived mesenchymal stem cells (AdMSC) were loaded with citric acid coated superparamagnetic iron oxide nanoparticles (SPIONs), systemically transplanted and magnetically recruited to the injured sciatic nerve. AdMSC arrival to the injured nerve was significantly increased using magnetic targeting and their beneficial effects surpassed the regenerative properties of the stand-alone cell therapy. AdMSC-SPIONs group showed a partially conserved nerve structure with many intact myelinated axons. Also, a very remarkable restoration in myelin basic protein organization, indicative of remyelination, was observed. This resulted in an improvement in nerve conduction, demonstrating functional recovery. In summary, our results demonstrate that magnetically assisted delivery of AdMSC, using a non-invasive and non-traumatic method, is a highly promising strategy to promote cell recruitment and sciatic nerve regeneration after traumatic injury. Last but not least, our results validate magnetic targeting in vivo exceeding previous reports in less complex models through cell magnetic targeting in vitro and ex vivo. This research was funded by CONICET (Grant numbers PIP11220170101059CO and 897), ANPCYT (Grant number PICT 3952), UNLP (Grant numbers X11/680 and X11/784), and UBACYT (Grant number 20020130100024). |
| Databáze: | OpenAIRE |
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