Sperm-associated antigen 11A is expressed exclusively in the principal cells of the mouse caput epididymis in an androgen-dependent manner
| Autor: | Dwi Ari Pujianto, Puji Sari, Yurnadi Hanafi Midoen, Purnomo Soeharso, Evelyn Loanda |
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| Rok vydání: | 2013 |
| Předmět: |
Male
Signal peptide Time Factors beta-Defensins medicine.drug_class Blotting Western Molecular Sequence Data Immunocytochemistry Spag11a Biology Androgen Mice Endocrinology medicine Animals Amino Acid Sequence Phosphorylation Principal cells Oligonucleotide Array Sequence Analysis Epididymis Binding Sites Base Sequence Reverse Transcriptase Polymerase Chain Reaction Research Gene Expression Profiling Obstetrics and Gynecology Efferent ducts Immunohistochemistry Spermatozoa Molecular biology Sperm Secretory protein Cell biology Sperm Maturation Gene expression profiling medicine.anatomical_structure Reproductive Medicine Androgens Orchiectomy Developmental Biology |
| Zdroj: | Reproductive Biology and Endocrinology : RB&E |
| ISSN: | 1477-7827 |
| Popis: | Background Epididymal sperm maturation occurs via interactions between sperm and proteins secreted by the epididymal epithelium. Although this is an important process, the genes that encode the involved proteins remain largely uncharacterized. Previous studies have demonstrated that the genes involved in sperm maturation are regulated by androgen. Spag11a is an epididymal gene that is influenced by androgen. However, little is known about the putative role of this gene in the sperm maturation process. The objective of this study was to characterize Spag11a in the mouse epididymis. Methods In silico analyses were performed to predict signal peptides and functional domains. Spag11a expression was measured by quantitative real-time RT-PCR. Western blots and immunocytochemistry were performed to determine protein expression. Results SPAG11A is a member of the beta defensin protein family and constitutes a secretory protein. Spag11a was expressed exclusively in the epididymis. Moreover, it exhibited region-specific expression in the caput, which is typical for genes that are involved in creating a suitable microenvironment for sperm maturation. Mouse Spag11a was regulated by androgen. A significant decrease of Spag11a expression was observed at third day following a gonadectomy (P |
| Databáze: | OpenAIRE |
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