Ceramide stimulates the uptake of neutral red in human neutrophils, monocytes, and lymphocytes
| Autor: | Sándor Sipka, I. Szöllösi, Istvan Csipo, Gyula Szegedi, Gabriella Lakos, Péter Antal-Szalmás |
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| Rok vydání: | 2000 |
| Předmět: |
Ceramide
Neutral red Neutrophils Phosphatidic Acids Arachidonic Acids Biology Klinikai orvostudományok Ceramides Monocytes chemistry.chemical_compound Extracellular medicine Staurosporine Humans Lymphocytes Enzyme Inhibitors Coloring Agents Protein kinase C Protein Kinase C Monocyte Hematology General Medicine Orvostudományok Cell biology medicine.anatomical_structure chemistry Neutral Red Sphingomyelin Intracellular medicine.drug |
| Zdroj: | Annals of hematology. 79(2) |
| ISSN: | 0939-5555 |
| Popis: | Neutral red is a vital stain known to be accumulated in the lysosomes of neutrophils and monocytes. It is used mainly to identify and detect the activated state of these cells. We have found that the extracellular application of physiological ceramide, i.e., a product of sphingomyelin hydrolysis and a newly defined intracellular second-messenger substance, increased the uptake of neutral red in a dose-dependent manner in human neutrophils, monocytes, and lymphocytes, as demonstrated by flow cytometry. Staurosporine was able to totally block this phenomenon, suggesting the involvement of protein kinase C in the process. These results indicate that the flow-cytometric analysis of ceramide-induced uptake of neutral red can be a new method for the evaluation of lysosome-related activation processes in both phagocytes and lymphocytes. |
| Databáze: | OpenAIRE |
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