Dietary sodium butyrate protects lipopolysaccharide-induced inflammatory response in lambs through inhibiting TLR4/NF-κB signalling pathway

Autor:	Jiahua Du, Xiang Chen, Chunyuan Pan, Ning Jiang, Xinyu Zhang, Zhao Wang, Bofu Zhang, Kexin Wang, Cunhao Zhang, Lijun Zuo, Gan Liu, Muyang Li, Ai-Zhong Zhang
Rok vydání:	2021
Předmět:	coated sodium butyrate musculoskeletal diseases congenital hereditary and neonatal diseases and abnormalities Lipopolysaccharide Inflammatory response lipopolysaccharide nutritional and metabolic diseases Sodium butyrate Butyrate Pharmacology SF1-1100 Hedgehog signaling pathway Animal culture intestinal function weaned lambs Basal (phylogenetics) chemistry.chemical_compound Dietary Sodium chemistry Tlr4 nf κb Animal Science and Zoology
Zdroj:	Italian Journal of Animal Science, Vol 20, Iss 1, Pp 1398-1409 (2021)
ISSN:	1828-051X
DOI:	10.1080/1828051x.2021.1955627
Popis:	This study evaluated the effects of dietary coated sodium butyrate (CSB) on lipopolysaccharide (LPS)-induced inflammatory response in weaned lambs. A basal diet was supplemented with CSB at 0, 2 or 3 g/kg feed for 28 days and/or intraperitoneally injected with 100 μg/kg BW Escherichia coli LPS (0 g/kg CSB, 0 g/kg CSB and LPS, 2 g/kg CSB and LPS, 3 g/kg CSB and LPS). The results showed that CSB supplementation conferred significant protective effects against LPS-induced inflammatory response by reducing in the levels of D-lactate (DLA), diamine oxidase (DAO), inflammatory cytokines (TNF-α, IL-1β and IL-8) in the serum and upregulated expression of tight junction proteins (claudin-3, ZO-1 and occludin) in the ileum. Meanwhile, CSB pre-treatment significantly suppressed LPS-induced TLR4/NF-κB signalling pathway mRNA expression (MyD88, TLR4, NF-κB, IL-6 and IL-1β) and down-regulated TLR4/NF-κB signalling pathway protein expression (MyD88, TLR4, NF-κB p65 and p-NF-κB p65). Furthermore, dietary CSB supplementation improved intestinal morphology. Lastly, bacterial 16S rRNA gene sequencing indicated that CSB could alter intestinal microbiota composition at genus levels. Taken together, our results indicate CSB could maintain intestinal health, improve intestinal morphology, modulate the microbial community and decrease LPS-induced inflammatory response in lambs.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::576b055e4d19355b25fca42a5f9dbb56 https://doi.org/10.1080/1828051x.2021.1955627 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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