Lentivirus-mediated PLCγ1 gene short-hairpin RNA suppresses tumor growth and metastasis of human gastric adenocarcinoma
| Autor: | Fen Wang, Bingchang Zhang, Heguo Cai, Chun Xia, Lianzhi Dai, Song Gang, Bing Zhang, Tianhui Hu, Yan-yan Zhan |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
MAPK/ERK pathway Oncology Fluorescent Antibody Technique Apoptosis Metastasis Small hairpin RNA Immunoenzyme Techniques Mice 0302 clinical medicine Nude mouse Cell Movement Targeted Molecular Therapy Tumor Cells Cultured RNA Small Interfering Mice Inbred BALB C biology Reverse Transcriptase Polymerase Chain Reaction Gene Expression Regulation Neoplastic tumor growth 030220 oncology & carcinogenesis ShRNA Adenocarcinoma Female Signal Transduction Research Paper medicine.medical_specialty PLCγ1 Blotting Western Mice Nude Real-Time Polymerase Chain Reaction 03 medical and health sciences Stomach Neoplasms Internal medicine medicine Animals Humans RNA Messenger human gastric adenocarcinoma cells Protein kinase B Cell Proliferation tumor metastasis Cell growth business.industry Phospholipase C gamma Lentivirus medicine.disease biology.organism_classification Xenograft Model Antitumor Assays digestive system diseases 030104 developmental biology business Proto-Oncogene Proteins c-akt |
| Zdroj: | Oncotarget |
| ISSN: | 1949-2553 |
| Popis: | // Bingchang Zhang 1 , Fen Wang 1 , Lianzhi Dai 1 , Heguo Cai 2 , Yanyan Zhan 1 , Song Gang 1 , Tianhui Hu 1 , Chun Xia 2 , Bing Zhang 1 1 School of Medicine, Xiamen University, Fujian, 361102, China 2 Zhongshan Hospital, Xiamen University, Fujian, 361004, China Correspondence to: Chun Xia, e-mail: chunxia@xmu.edu.cn Bing Zhang, e-mail: cristal66@xmu.edu.cn Keywords: PLCγ1, ShRNA, tumor growth, tumor metastasis, human gastric adenocarcinoma cells Received: September 09, 2015 Accepted: January 01, 2016 Published: January 22, 2016 ABSTRACT Targeted molecular therapy has gradually been a potential solution in cancer therapy. Other authors’ and our previous studies have demonstrated that phosphoinositide-specific phospholipase γ (PLCγ) is involved in regulating tumor growth and metastasis. However, the molecular mechanism underlying PLCγ-dependent tumor growth and metastasis of gastric adenocarcinoma and whether PLCγ may be a potential target for tumor therapy in human gastric adenocarcinoma are not yet well determined. Here, we investigated the role of PLCγ inhibition in tumor growth and metastasis of human gastric adenocarcinoma using BGC-823 cell line and a nude mouse tumor xenograft model. The results manifested that the depletion of PLCγ1 by the transduction with lentivirus-mediated PLCγ1 gene short-hairpin RNA (shRNA) vector led to the decrease of tumor growth and metastasis of human gastric adenocarcinoma in vitro and in vivo . Furthermore, the Akt/Bad, Akt/S6, and ERK/Bad signal axes were involved in PLCγ1-mediated tumor growth and metastasis of human gastric adenocarcinoma. Therefore, the abrogation of PLCγ1 signaling by shRNA could efficaciously suppress human gastric adenocarcinoma tumor growth and metastasis, with important implication for validating PLCγ1 as a potential target for human gastric adenocarcinoma. |
| Databáze: | OpenAIRE |
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