Small Ultra-Red Fluorescent Protein Nanoparticles as Exogenous Probes for Noninvasive Tumor Imaging In Vivo
| Autor: | Nandi Chen, Jingqi Xin, Erik A. Rodriguez, William J. Conlon, Feifei An, Justin S. Hachey, Richard Ting, Omer Aras |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Lung Neoplasms
Chemical structure Nanoparticle Mice Nude 02 engineering and technology Biochemistry Article Green fluorescent protein 03 medical and health sciences chemistry.chemical_compound Mice Structural Biology In vivo Animals Humans Cyanine Bovine serum albumin Molecular Biology 030304 developmental biology Fluorescent Dyes 0303 health sciences Mice Inbred BALB C biology Chemistry Optical Imaging General Medicine 021001 nanoscience & nanotechnology Fluorescence Luminescent Proteins A549 Cells biology.protein Biophysics Heterografts Nanoparticles 0210 nano-technology Preclinical imaging Neoplasm Transplantation |
| Zdroj: | Int J Biol Macromol |
| Popis: | Nanoparticles are excellent imaging agents for cancer, but variability in chemical structure, racemic mixtures, and addition of heavy metals hinders FDA approval in the United States. We developed a sm all u ltra- r ed f luorescent p rotein, named smURFP, to have optical properties similar to the small-molecule Cy5, a heptamethine subclass of cyanine dyes (Ex/Em = 642/670 nm). smURFP has a fluorescence quantum yield of 18% and expresses so well in E. coli, that gram quantities of fluorescent protein are purified from cultures in the laboratory. In this research, the fluorescent protein smURFP was combined with bovine serum albumin into fluorescent protein nanoparticles. These nanoparticles are fluorescent with a quantum yield of 17% and 12–14 nm in diameter. The far-red fluorescent protein nanoparticles noninvasively image tumors in living mice via the enhanced permeation and retention (EPR) mechanism. This manuscript describes the use of a new fluorescent protein nanoparticle for in vivo fluorescent imaging. This protein nanoparticle core should prove useful as a biomacromolecular scaffold, which could bear extended chemical modifications for studies, such as the in vivo imaging of fluorescent protein nanoparticles targeted to primary and metastatic cancer, theranostic treatment, and/or dual-modality imaging with positron emission tomography for entire human imaging. |
| Databáze: | OpenAIRE |
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