In Vitro and In Vivo Studies of Hydrophilic Electrospun PLA95/β-TCP Membranes for Guided Tissue Regeneration (GTR) Applications
| Autor: | Jen Chang Yang, Sheng-Yang Lee, Chien-Chung Chen, Hsin-Tai Hu, Nai-Chia Teng, Pei-Chi Huang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
PLA95
Biocompatibility General Chemical Engineering 0206 medical engineering 02 engineering and technology Article lcsh:Chemistry biocompatibility In vivo medicine Periodontal fiber General Materials Science Cementum Dental alveolus electrospinning guided tissue regeneration (GTR) Chemistry Soft tissue 021001 nanoscience & nanotechnology 020601 biomedical engineering In vitro Membrane medicine.anatomical_structure lcsh:QD1-999 0210 nano-technology Biomedical engineering |
| Zdroj: | Nanomaterials Volume 9 Issue 4 Nanomaterials, Vol 9, Iss 4, p 599 (2019) |
| ISSN: | 2079-4991 |
| DOI: | 10.3390/nano9040599 |
| Popis: | The guided tissue regeneration (GTR) membrane is a barrier intended to maintain a space for alveolar bone and periodontal ligament tissue regeneration but prevent the migration of fast-growing soft tissue into the defect sites. This study evaluated the physical properties, in vivo animal study, and clinical efficacy of hydrophilic PLA95/&beta TCP GTR membranes prepared by electrospinning (ES). The morphology and cytotoxicity of ES PLA95/&beta TCP membranes were evaluated by SEM and 3-(4,5-cimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) respectively. The cementum and bone height were measured by an animal study at 8 and 16 weeks after surgery. Fifteen periodontal patients were selected for the clinical trial by using a commercial product and the ES PLA95/&beta TCP membrane. Radiographs and various indexes were measured six months before and after surgery. The average fiber diameter for this ES PLA95/&beta TCP membrane was 2.37 ± 0.86 µ m. The MTT result for the ES PLA95/&beta TCP membrane showed negative for cytotoxicity. The significant differences in the cementum and bone height were observed between empty control and the ES PLA95/&beta TCP membrane in the animal model (p < 0.05). Clinical trial results showed clinical attachment level (CAL) of both control and ES PLA95/&beta TCP groups, with a significant difference from the pre-surgery results after six months. This study demonstrated that the ES PLA95/&beta TCP membrane can be used as an alternative GTR membrane for clinical applications. |
| Databáze: | OpenAIRE |
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